成人隐匿性自身免疫性糖尿病患者CD_4^+CD_(25)^+ T细胞亚群的变化  

Changes of CD_4^+CD_(25)^+ T cell subsets in LADA patients

作　　者：何凌[1,2] 周智广[1] 罗建华[1] 杨琳[1] 陈小燕[1] 王建平[1] 李莉蓉[1] 邓志明[1] 苏恒[1] 

机构地区：[1]中南大学湘雅二医院内分泌科,中南大学代谢内分泌研究所,中南大学糖尿病中心,长沙现在410011 [2]广州市第一人民医院内分泌科

出　　处：《中华糖尿病杂志（1006-6187）》2005年第6期428-431,共4页

基　　金：国家自然科学基金资助项目(30370681);教育部"跨世纪优秀人才"基金资助项目(2002-48);湖南省卫生厅重点资助项目(2001-Z04);湖南省科技厅重点资助项目(03SSY1009)

摘　　要：目的探讨成人隐匿性自身免疫性糖尿病(LADA)患者外周血免疫调节性CD4+CD25+T细胞亚群的变化及其意义。方法以流式细胞仪检测32例LADA、16例T1DM、25例T2DM及27例正常对照外周血CD4+CD25+、CD3+CD8+T细胞。结果①LADA组外周血CD4+CD25+T细胞低于而CD3+CD8+T细胞高于T1DM组、T2DM组和正常对照组;②LADA患者CD3+CD8+T细胞比例与起病年龄、FC-P、2hC-P呈负相关。结论LADA患者免疫调节性CD4+CD25+T细胞减少,不能有效维持对胰岛自身抗原的耐受;细胞毒性CD3+CD8+T细胞增多从而破坏胰岛β细胞,导致自身免疫性糖尿病的发生和发展。Objective To investigate the frequency and etiopathogenetic relevance of CD4^ ＋ CD25^ ＋ immunoregulatory T cells in latent autoimrnune diabetes in adults （LADA）. Methods Peripheral blood samples were obtained from patients with 32 LADA, 16 type 1 diabetes, 25 type 2 diabetes and 27 controls. The frequencies of peripheral CD 4^＋ CD 25^＋ double-positive, CD3^＋ CD a ＋ double-positive cells were measured by flowcytometry. Results ① We found lower numbers of CD 4^＋ CD25^＋ T cells in LADA patients than those in T1DM, T2DM and control groups （0.71% vs 1.20%, 1.26%, 1.27%）. ②Higher frequencies of CD 3^＋ CD8^＋ T cells were found in LADA group than in T1DM,T2DM and control ones （35.10% vs 24. 40%, 23. 55%, 22. 15%）.③ The frequency of CD3^＋ CD8^＋ T cells was negatively correlated with age at onset, fasting and postprandial C-peptides. Conclusions The frequency of CD 4^＋ CD 25^ ＋ T cells decreases in LADA patients, which cannot maintain the tolerance to islet autoantigens effectively. Cytotoxic CD3^＋ CD8^＋ T cells in the periphery blood simultaneously increase to attack and destroy islet β cells, of autoimmune diabetes.

关 键 词：成人隐匿性自身免疫性糖尿病 CD4^+CD25^+T细胞 CD3^+CD8^+T细胞 

分 类 号：R587.1[医药卫生—内分泌] R512.62[医药卫生—内科学]