人类大肠癌SSTR2 mRNA表达及其与细胞增殖相关性研究  

SSTR2 mRNA expression and correlation with proliferating cell nuclear antigen in human colorectal cancer

作　　者：代远斌[1] 曾希志[1] 向志[1] 

机构地区：[1]重庆医科大学附属第一医院普通外科,重庆400016

出　　处：《第三军医大学学报》2005年第23期2363-2365,共3页Journal of Third Military Medical University

摘　　要：目的了解人类大肠癌组织中生长抑素受体亚型2(SSTR2)mRNA的表达情况,并探讨其与细胞增殖的关系。方法收集60例大肠癌标本,采用多相寡核苷探针原位杂交法检测SSTR2mRNA表达,免疫组化法检测增殖细胞核抗原(PCNA)表达,并用图像分析仪进行SSTR2mRNA相对含量测定。结果SSTR2mRNA在早期和分化好的大肠癌表达的阳性率和相对含量均明显高于晚期和分化差的病变,并发现大肠癌SSTR2mRNA表达与PCNA表达呈负相关(P<0·05)。结论SSTR2mRNA表达与大肠癌的分化程度和临床分期有关;SSTR2mRNA是阻滞肿瘤细胞增殖的因素之一,对大肠癌具有反映恶性程度及估计预后的价值。Objective To investigate somatostatin receptor subtype 2 （SSTR2） mRNA expression and its correlation with proliferating cell nuclear antigen （PCNA） in human colorectal cancer. Methods A total of 60 colorectal cancer samples were analyzed. The SSTR2 mRNA expression was examined by in situ hybridization （ISH） using muhiphase oligoprobes. The PCNA were detected by immunohistochemical staining. A computerized image analysis system was utilized to estimate the relative contents of SSTR2 mRNA. Results The positive rate of expression of SSTR2 mRNA and relative contents in early and well-differentiated colorectal cancer were higher than those in advanced and poorly differentiated lesion. A negative correlation between SSTR2 mRNA expression and PCNA was found in colorectal cancer. Conclusion In the samples of colorectal cancer, the expression of SSTR2 mRNA is correlated with the degree of differentiation and clinical stage, and it plays an important role in reflecting the degree of malignancy and prognosis.

关 键 词：大肠癌 生长抑素受体 增殖细胞核抗原 SSTR2 MRNA 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R735.34[医药卫生—基础医学]