单细胞凝胶电泳检测大鼠死后肝细胞核DNA降解  

A study on the relationship between nuclear DNA degradation of the rat's liver cells and the postmortem interval by using single cell gel electrophoresis

作　　者：何远[1] 闫平[1] 胡家伟[2] 孟晓萍[1] 

机构地区：[1]武汉大学医学院法医学教研室,湖北武汉430071 [2]武汉市公安局刑侦处,湖北武汉430019

出　　处：《中国法医学杂志》2005年第6期325-328,共4页Chinese Journal of Forensic Medicine

摘　　要：目的应用单细胞凝胶电泳技术(SCGE)检测大鼠死后肝细胞核DNA降解规律,分析与死亡时间的关系,为早期死亡时间的推断提供新的方法.方法在大鼠死后30h内,每隔3h取肝组织样本进行单细胞凝胶电泳,用共聚焦显微镜摄取彗星图像,应用彗星图像分析软件(IMI1.0)进行图像分析,并作统计学分析.结果死后大鼠的肝细胞在电泳图像上出现明显的彗星形拖尾,其尾长(TL)、尾矩(TM)在一定的时间范围内(0～18h)随死亡时间的延长而逐渐增大,二者均与死亡时间(PMI)呈现一定的相关回归关系.结论单细胞凝胶电泳技术可应用于早期死亡时间的推断.Objective To explore the liver nuclear DNA degradation in rats after death by single cell gel eleetrophoresis and to provide a new method for the estimation of early postmortem interval. Methods A rat death model was established. The samples of liver were taken at 3 - hour - interval within 30 hours after the rat＇ s death. After single cell gel eleetrophoresis, the comet images were captured by the eonfoeal microscope and analyzed by SCGE image analysis software（IMI1.0）. Results An evident ＇ comet tail＇ was observed after SCGE Assay. The ＇ tail length＇ and the ＇ tail moment＇ increased with an increased PMI （0 - 18h） ,which shows strong statistical correlation with PMI. Conclusion The SCGE Assay of liver nuclear DNA degradation may be used as a predictor of early postmortem interval.

关 键 词：法医病理学 死亡时间 DNA降解 单细胞凝胶电泳 彗星图像分析软件 大鼠 肝细胞 

分 类 号：D919[医药卫生—法医学] R811.5[政治法律—法学]