维库溴铵对大鼠膈肌神经肌肉接头微终板电位和终板电位幅度和频率的影响  

Effects of vecuronium on neuromuscular junction miniature endplate potentials and endplate potentials in rat diaphragm

作　　者：李军[1] 刘永勤[1] 张宏[2] 

机构地区：[1]海军总医院麻醉科,北京100037 [2]解放军总医院麻醉科,北京100853

出　　处：《第二军医大学学报》2005年第12期1374-1376,共3页Academic Journal of Second Military Medical University

基　　金：军队"九五"面上项目资助课题(98D061)

摘　　要：目的:利用成年大鼠膈肌膈神经不均匀牵拉标本,研究维库溴铵对神经肌肉接头部位的电生理学影响。方法:快速处死成年健康Wistar大鼠制备离体大鼠不均匀牵张膈肌膈神经标本(INSMP);记录到正常微终板电位(MEPP)和终板电位(EPP)后,观察维库溴铵在相当于大鼠4×ED95(4μg/ml)、2×ED95(2μg/ml)、1×ED95(1μg/ml)和0.5×ED95(0.5μg/ml)的剂量时对MEPP频率和幅度的影响。结果:维库溴铵在0.5μg/ml浓度时对正常大鼠离体不均匀牵张膈肌膈神经(INSMP)上的MEPP的频率、幅度以及EPP幅度无明显影响;在1μg/ml剂量时,给药后5 min始对MEPP频率、幅度以及EPP幅度产生明显抑制作用(P<0.05);但在给药后10 min,维库溴铵4μg/ml也未能完全抑制MEPP的产生。结论:维库溴铵能减少突触前递质的释放。Objective：To investigate the effects of vecuronium on the electrophysiology in neuromuscular junction of rat diaphragm in an isolated non-uniform stretched muscle preparation（INSMP）. Methods： The INSMP was made in healthy adult rats which were sacrificed rapidly. The amplitude and frequency of miniature endplate potentials （MEPP） and endplate potentials （EPP） were recorded before and after the INSMP was treated with vecuronium at 4×ED95（4μg/ml）、2×ED95（2μg/ml）、1×ED95（1μg/ml） and 0.5×ED95（0.5μg/ml） separately. Results： Vocuronium at 0.5μg/ml had no obvious effect on the amplitude and frequency of MEPP and the amplitude of EPP, but vocuronium at 1μg/ml had significant inhibitive effects on the amplitude and frequency of MEPP and the amplitude of EPP 5 rain after treatment （P〈0.05）. Vocuronium at 4.0μg/ml did not completely inhibit MEPP even 10 min after treatment. Conclusion： The results indicate that vecuronium might decrease the presynaptic neurotransmitter release in neuromuscular junction of rats.

关 键 词：维库溴铵 终板电位 微终板电位 膈神经 

分 类 号：R614[医药卫生—麻醉学]