人骨生物衍生材料制备的实验研究  

Experimental Research on Preparation of Natural Derived Material from Human Bone

作　　者：康非吾[1] 唐休发[2] 温玉明[2] 王佐林[1] 潘可风[1] 

机构地区：[1]同济大学口腔医学院口腔颌面外科,上海200072 [2]四川大学华西口腔医院口腔颌面外科,成都610041

出　　处：《同济大学学报（医学版）》2005年第6期4-7,共4页Journal of Tongji University(Medical Science)

基　　金：上海市卫生局科技发展基金资助项目(044106)

摘　　要：目的探索人脱蛋白骨及脱钙骨的制备方法,并以HA陶瓷为对照,对制得的骨生物衍生材料的形貌特征、组成成份等进行分析。为研究其用作骨组织工程的支架材料的可行性打下基础。方法通过物理或化学的方法,对人骨经去抗原、脱细胞、脱蛋白、脱钙等处理制备人骨生物衍生支架材料。结果骨生物衍生材料(脱蛋白骨、脱钙骨)具有原骨组织天然的三维网状孔隙系统;脱蛋白骨的主要成份为羟基磷灰石,脱钙骨的主要成份为胶原,其Ca、P含量几乎为零。结论本实验通过对人股骨脱细胞、脱脂肪、脱蛋白和脱钙等处理,建立了骨生物衍生材料(脱蛋白骨、脱钙骨)的制备方法,为进一步研究其在骨组织工程中大应用打下基础。Objective To explore a new method for preparation of natural derived material from human bone and to study its morphological features and constitute components for laying foundation for further clinical application. Methods Human thigh bone was deproteinated and decalcified. Its mophological features and constitute components were detected by scanning electron microscoppe, X-ray and diffraction analysis, etc.. Results It showed that the natural derived material maintained the natural network proe system; the main components of deproteinated and decalcified bone were carbonated hydroxyapatite and collagen. Conclusion A preparation of natural derived material is obtained and it characteristics and future clinical application are studied and discussed.

关 键 词：生物衍生材料 组织工程 材料制备 HA陶瓷 人脱蛋白骨 脱钙骨 

分 类 号：R318.17[医药卫生—生物医学工程]