茜草素的抗菌活性与药效试验  

Antibacterial activity and curative effect of alizarin

作　　者：崔颖[1] 梁剑平[1] 陈积红 牛建荣[1] 李宏胜[1] 吕佳文[1] 王学红[1] 华兰英[1] 郭延生[3] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所农业部新兽药工程重点开放实验室,甘肃兰州730050 [2]中牧股份兰州生物药厂,甘肃兰州730046 [3]甘肃农业大学动物医学院,甘肃兰州730070

出　　处：《中国兽医科技》2005年第12期1008-1011,共4页Chinese Journal of Veterinary Science and Technology

基　　金：国家"十五"科技攻关项目(2002BA514A-17-07)

摘　　要：用化学合成的茜草素做了体外抑菌试验、体内抗菌活性试验、人工感染猪多杀巴氏杆菌疗效试验、急性毒性试验及临床治疗试验。结果表明,茜草素在体外对金黄色葡萄球菌、变形杆菌、大肠埃希氏菌、绿脓杆菌、沙门菌和克雷伯氏菌等均有显著的抑制作用,对后4种病原菌的抑制作用超过了六茜素。在小白鼠体内对大肠埃希氏菌也有非常显著的抗菌作用。对人工感染多杀巴氏杆菌的患病仔猪有显著的治疗作用。小白鼠腹腔注射茜草素的LD50为336 mg/kg。茜草素对临床型乳牛乳腺炎的治愈率为87.5%,总有效率为100%。Inhibitory test in vitro, antibacterial activity test in vivo, treatment test for pigs infected with Pasteurella multocida, acute toxicity test and clinical treatment test were carried out to evaluate the antibacterial activity and curative effect of alizarin. The inhibitory test in vitro showed that alizarin had remarkable inhibitory effect on Staphylococcus aureus, Proteus, Escherichia coli, Pseudomonas aeruginosa, Salmonella and Klebsiella in vitro, and had stronger inhibitory effect than pyroline on E. coli, P. aeruginosa, Salmonella and Klebsiella. The antibacterial activity test indicated that alizarin had remarkable antibacterial activity against E. coli in mice in vivo. The treatment test in pigs with pasteurellosis showed that alizarin had curative effect on piglets infected with P. multocida. The acute toxicity test and clinical treatment test showed that LD50 of mice injected with alizarin was 336 mg/kg, and the rate of recovery and total validity to clinical type of dairy cow＇s mastitis were 87.5% and 100% respectively.

关 键 词：茜草素 六茜素 猪多杀巴氏杆菌病 乳腺炎 

分 类 号：S853.7[农业科学—临床兽医学]