Gαq/11在哇巴因信号转导中的意义  

The significance of Gαq/11 protein in ouabain signal transduction

作　　者：梁磊[1] 艾文婷[1] 吕卓人[1] 

机构地区：[1]西安交通大学第一医院老年心内科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2005年第6期533-535,540,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的观察不同浓度哇巴因对SD大鼠胸主动脉平滑肌细胞Gαq/11表达的影响,并探讨其在哇巴因信号转导中的作用。方法采用贴块法原代培养SD大鼠胸主动脉平滑肌细胞,进行传代和纯化。分别给予生理浓度以及病理浓度的哇巴因干预48 h,免疫细胞化学染色观察平滑肌细胞Gαq/11蛋白的表达。结果生理浓度哇巴因干预后,平滑肌细胞有增殖现象;而病理浓度组细胞发生凋亡。生理浓度组Gαq/11表达(111.21±30.47)显著高于病理浓度组(68.70±6.21)以及对照组(58.81±4.51,P<0.01);病理浓度组和对照组相比无显著变化。结论①Gαq/11介导了生理浓度哇巴因引起细胞增殖的生物学效应;②病理浓度哇巴因引起细胞凋亡的生物学效应与Gαq/11无关;③哇巴因在不同浓度下,可能通过不同的信号转导通路产生相应的药理学作用或生物学效应。Objective To explore the changes of Gαq/11 expression in smooth muscle cells and its role in ouabain cell signal transduction, Methods The Gαq/11 was analyzed with immunohistochemistry after the primary culture of Sprague-Dawley rats thoracic aorta smooth muscle cells were treated with different concentrations of ouabain for 48 hours. Results The expressions of Gαq/11 protein in the physiological concentration group （111.21 ± 30,47） were significantly higher than those in the pathological concentration group （68.70± 6.21） and the control group （58.81± 4.51, P〈0.01）. There was no significant difference between the pathological concentration group and the control group. Conclusion The proliferation of smooth muscle cells induced by ouabain in physiological concentration is mediated by Gαq/11. The apoptosis of smooth muscle cells induced by ouabain in pathological concentration is not related with Gαq/11. Ouabain might induce different pharmacological or biological effects through different signal transduction pathways.

关 键 词：哇巴因 信号转导通路 Gαq/11蛋白 平滑肌细胞增殖 

分 类 号：R334.1[医药卫生—人体生理学]