β-淀粉样蛋白增加基底前脑神经元对谷氨酸的易感性  

Study on beta amyloid protein enhancing the sensibility of primary cultured basal forebrain neuron to glutamic acid

作　　者：钱亦华[1] 王晓玲[2] 朱淑娟[1] 史利利[1] 胡明[1] 刘勇[1] 胡晓丹[1] 韩华[1] 

机构地区：[1]西安交通大学医学院人体解剖与组织胚胎学系 [2]天津中医学院组织胚胎学教研室,天津300193

出　　处：《西安交通大学学报（医学版）》2005年第6期536-540,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：西安市科技计划项目(No.GG04145)

摘　　要：目的探讨β-淀粉样蛋白25-35(Aβ25-35)与谷氨酸(Glu)联合应用,对原代培养的大鼠基底前脑神经元的作用。方法原代培养大鼠基底前脑神经元,应用形态学、MTT法结合ChAT免疫组织化学染色,分别观察Aβ25-35与Glu联合应用,对原代培养的基底前脑神经元存活率、形态学及ChAT免疫反应阳性神经元的影响。结果将100 nmol/L、1μmol/L Aβ分别和20μmol/L Glu联合用于培养的基底前脑神经元,在倒置显微镜下观察,细胞表面粗糙,立体感减弱,胞浆中出现深色颗粒,细胞肿胀或收缩;尼氏染色显示尼氏体减少;MTT检测显示神经元存活率明显下降,100nmol/L Aβ+20μmol/L Glu组与100 nmol/L Aβ组间,1μmol/L Aβ+20μmol/L Glu组与1μmol/L Aβ组间比较均具有统计学差异(P<0.05);ChAT免疫阳性神经元的灰度和截面积均减小。结论Aβ25-35可增加神经元对Glu神经毒性作用的敏感性,表明Glu在阿尔茨海默病发病中起着非常关键的作用。Objective To study the effects of Aβ025-35 and glutamic acid on primary cultured rat basal forebrain neurons. Methods Morphological study, MTT test, and ChAT immunohistochemistry method were adopted to observe and analyze the effects of Aβ25-35 and glutamic acid on morphology, survival rate, choline acetylase positive neurons of rat basal forebrain, Results 100 nmol/L and 20μmol/L glutamic acid either 1μmol/L Aβ25-35 and 20μmol/L glutamic acid were added to medium, respectively, under inverted microscope, the surface of neurons were rough, stereognosis of neurons died down, the dark granules-like appeared in perikaryon; Nissl bodys decreased, the neurons were swollen or contracted in Nissl staining;the result of MTT test showed that the survival rate of neurons was obviously decreased. There were differences between 100 nmol/L Aβ ＋20μmol/L Glu group and 100 nmol/L Aβ group, 1μmol/L Aβ ＋ 20μmol/L Glu group and 1 μmol/L Aβ （ P〈0.05）, respectively, The optic density and area of ChAT positive neurons was deminished. Conclusion Aβ25-35 can enhance the sensibility of primary cultured neurons of rat basal forebrain to glutamic acid, Glutamic acid play a key role in the pathological process of Alzheimer＇s disease.

关 键 词：Β-淀粉样蛋白 谷氨酸 基底前脑 细胞培养 大鼠 

分 类 号：R332.81[医药卫生—人体生理学]