骨髓基质干细胞复合小肠黏膜下层体外构建组织工程骨膜的实验研究  

Periosteum construction in vitro by small intestinal submucosa combined with bone marrow mesenchymal stem cell

作　　者：张开刚[1] 曾炳芳[1] 张长青[1] 

机构地区：[1]上海交通大学附属第六人民医院骨科,200233

出　　处：《中华外科杂志》2005年第24期1594-1597,共4页Chinese Journal of Surgery

基　　金：国家自然科学基金资助项目(30371444)

摘　　要：目的探讨用猪小肠粘膜下层(SIS)作支架,复合骨髓间充质干细胞(BMSC)体外构建组织工程骨膜的可行性。方法用常规方法培养BMSC,再与SIS进行体外复合培养,分别进行相差显微镜、透射电镜、扫描电镜和组织学检查。观察BMSC在SIS上的生长、分化、增殖及细胞分泌细胞外基质情况。结果BMSC在SIS材料上粘附、增殖,分泌大量的细胞外基质成份,细胞功能活跃,在SIS上成多层生长,厚度随复合培养时间的延长而增加,类似生物骨膜。结论SIS与BMSC在体外复合培养可以构建出类似生物骨膜的组织工程骨膜,为进一步研究体内成骨奠定基础。Objective To investigate the practicability of periosteum construction in vitro by small intestinal submucosa （SIS） as a tissue scaffold with bone marrow mesenchymal stem cells （BMSC）. Methods The bone marrow mesenchymal stem cells were cultivated by the conventional method in vitro, and then co-cultivated with SIS. The morphological feature of the complex material , the extracellular matrices, the adhesion and proliferation of BMSC were observed by optical microscope, electronic microscope, scanning electronic microscope and histologic evaluation respectively. Results BMSCs adhered and proliferated on SIS, secreted a great deal of extracellular matrices with active cellular function. BMSCs grew well there in layers, and the thickness became thicker as time passed, acting like a biological periosteum. Conclusion Combined culture of SIS and BMSC in vitro can construct a tissue engineering periosteum similar to the natural one, thus providing a base for further study of its osteogenic regeneration in vivo.

关 键 词：干细胞 骨髓 小肠粘膜下层 组织工程 骨膜 

分 类 号：R318.0[医药卫生—生物医学工程]