血管紧张素转换酶2在SARS-CoV感染中的功能(第1部分)——血管紧张素转换酶2基因剔除小鼠的建立  

The Role of Converting Enzyme 2 in SARS-CoV Infection(Part Ⅰ)— Establishment of Angiotensin-Converting Enzyme 2 Knockout Mouse

作　　者：秦川[1] 刘先菊[1] 沈洁[1] 董伟[1] 张扬清[1] 宋明晶[1] 邸冉[1] 张连峰[1] 

机构地区：[1]中国医学科学院中国协和医科大学实验动物研究所遗传中心,北京100021

出　　处：《中国比较医学杂志》2005年第6期327-329,共3页Chinese Journal of Comparative Medicine

摘　　要：目的血管紧张素转换酶是肾素-血管紧张素系统的重要组成部分。血管紧张素转换酶主要功能是通过把血管紧张素Ⅰ转换成血管紧张素Ⅱ来调节血压,最新的研究显示它也是SARS冠状病毒的一种受体。建立血管紧张素转换酶2基因剔除小鼠,为进一步的SARS机理研究提供工具。方法通过将D3小鼠胚胎干细胞DNA进行同源重组,用反向表达的新霉素基因替代ACE2基因-2000 bp至+489 bp的DNA片段,并用Western blot检测纯合体小鼠ACE2基因的表达。结果血管紧张素转换酶2基因剔除小鼠ACE2基因的表达被完全阻遏。结论建立了ACE2基因剔除小鼠。Objective Angiotensin-converting enzyme 2 plays a central role in the rennin-angiotensin system through generation of the peptide angiotensin Ⅱ from its inactive precursor angiotensin Ⅰ. It is also the main receptor of SARS-CoV. The purpose of this study is to establish an angiotensin-converting enzyme 2 knockout mouse. Methods To establish that enzyme 2 knockout mouse through targeted homologous recombination in D3 ES cells. The - 2000 bp to ＋ 489 bp DNA fragment of ACE2 gene was replaced with a neomycin cassette. Results Western blot analysis indicated that the expression of ACE2 is completely blocked. Conehtsion An angiotensin-converting enzyme 2 knockout mouse has been established.

关 键 词：基因剔除 ACE2 小鼠 

分 类 号：R373[医药卫生—病原生物学]