大鼠脑出血早期脑组织神经元型一氧化氮合酶mRNA表达及中药抵当汤干预对其的影响  

Expression of nNOS mRNA of brain tissue at early stage of intracerebral hemorrhage in rats and the intervention effect of Didangtang

作　　者：刘韶华[1] 吴颢昕[2] 姜亚军[3] 周红[1] 

机构地区：[1]东南大学附属中大医院神经内科,南京210009 [2]南京中医药大学基础医学院 [3]南京中医药大学附属江苏省中医院脑病中心

出　　处：《临床神经病学杂志》2005年第6期436-439,共4页Journal of Clinical Neurology

基　　金：江苏省社会发展基金(BS200082)

摘　　要：目的探讨大鼠脑出血(ICH)早期脑组织神经元型一氧化氮合酶(nNOS)mRNA表达,以及中药抵当汤干预对其的影响。方法将72只大鼠随机分为ICH组、抵当汤组、NOS抑制剂组和对照组,采用立体定向技术注入自体不凝血建立实验性ICH模型,对照组注入等量的生理盐水;术后6h、24h及72h用Bederson3级标准法对大鼠进行神经功能障碍评定;然后断头取脑,以原位杂交方法检测脑组织中nNOSmRNA阳性细胞的表达。结果神经功能缺损程度评分术后72h时ICH组与抵当汤组均见明显改善(均P<0.05),但抵当汤组的改善明显优于ICH组(P<0.05);ICH后6h血肿侧大脑皮质nNOSmRNA阳性细胞的表达较对照组已有明显增高,24h达高峰,72h时下降,但仍明显高于对照组(均P<0.05);抵当汤组血肿侧大脑皮质nNOSmRNA阳性细胞表达在各时间点较ICH组明显减少(均P<0.05),且与NOS抑制剂组变化规律一致。结论抵当汤具有与NOS抑制剂相似的作用,通过抑制ICH后脑组织中nNOSmRNA的表达而产生脑保护作用。Objective To investigate the expression of neuron nitric oxide synthase （nNOS） mRNA of brain tissue at early stage of intracerebral hemorrhage （ICH） in rats and the intervention effect of Didangtang. Methods 72 male Spragne-Dawley rats were randomly divided into four groups： intracerebral hemorrhage group, Didangtang treatment group, NOS inhibition group and normal saline group. Quantitate fresh autologous blood was infused into fight caudate nucleus of rat sterotaxically to build up experimental ICH model and normal saline was instead in control group. The neurological function deficit scores were observed by Bederson method （3 grades） at 6 h, 24 h and 72 h after operation, At the same time points, the rats were sacrificed and the brain tissues were taken out for the measurement of nNOS mRNA by technique of hybridization in situ. Results Neurological function deficit scores of the rats were significantly improved both in intracerebral hemorrhage group and Didangtang treatment group at 72 h after operation（all P 〈 0. 05 ）. Further more, the scores in Didangtang group were markedly reduced than those in ICH group （P 〈 0. 05）. The expression of nNOS mRNA in the region of pallium ipsilateral to hemotoma in ICH rats increased at 6 h, peaked at 24 h, and decreased at 72 h, but also higher than normal saline group （ all P 〈 0. 05）. The expression of nNOS mRNA was significantly decreased in Didangtang group （ all P 〈 0. 05 ） , which was similar to NOS inhibition group. Conclusion Didangtang may play neuroprotective effect, and its mechanism may be related to inhibiting the expression of nNOS mRNA just as NOS inhibition.

关 键 词：脑出血 神经元型一氧化氮合酶 原位杂交 抵当汤 

分 类 号：R285[医药卫生—中药学]