慢性体外反搏对高胆固醇血症猪动脉内皮细胞的影响  

Effect of chronic enhanced external counterpulsation on arterial endothelial cells of porcine with hypercholesteremia

作　　者：陈小林[1] 何小洪[1] 张焰[1] 钱月桃[1] 梁陆光[1] 方典秋[1] 詹澄扬[1] 郑振声[1] 马虹[1] 

机构地区：[1]中山大学附属第一医院心内科,广东广州510089

出　　处：《第一军医大学学报》2005年第12期1491-1493,共3页Journal of First Military Medical University

基　　金：国家"十五"科技攻关项目(2001BA706B07);国家自然科学基金(30127001)~~

摘　　要：目的探讨慢性增强型体外反博(EECP)能否在体修复高胆固醇血症引起的猪动脉内皮细胞(ECs)损伤。方法高胆固醇血症猪反搏36h后,取前降支进行扫描电镜检查,收集胸主动脉ECs进行蛋白质组学检查。结果高脂组冠脉ECs排列不规则、大量脱落、斑块形成,反搏组ECs呈流线型排列、脱落程度显著减轻;与高脂组相比,在反搏组的ECs检测到6种高表达的蛋白。结论慢性EECP可能通过调节ECs蛋白表达,改善ECs粘附性和代谢及减轻ECs内皮凋亡,从而修复高胆固醇血症对动脉内皮形态和功能的损伤。Objective To verify whether chronic enhanced external counterpulsation （EECP） may repair artery endothelial cells （ECs） damage resulted from hypercholesteremia in pigs. Methods EECP was performed for 36 hours in pigs with hypercholesteremia and the left descending artery （LDA） was isolated for scanning electron microscopy （SEM）. The ECs were collected from the thoracic aorta and analyzed by proteomic technique. Results The ECs ofhypercholesteremia pigs showed irregular arrangement with obvious desquamation of coronary ECs and formation of atherosclerotic plaque, and these lesions were milder in EECP-treated pigs. Six over-expressed proteins were detected in the endothelial cells in EECP-treated pigs in comparison with those of the hypercholesteremia pigs. Conclusion Chronic EECP helps restore cell morphology and repair functional damage of ECs resulted from hypercholesteremia by regulating endothelial protein expressions, and consequently improves cell adhesion and intracellular metabolism and reducesg EC apoptosis.

关 键 词：增强体外反搏 高胆同醇血症 内皮细胞 蛋白质组学 形态学 

分 类 号：R589.2[医药卫生—内分泌]