HPLC-ELSD法测定苦杏仁中苦杏仁苷含量的研究  

Determination of amygdalin content in Semen Armeniacae Amarum by high-performance liquid chromatography with evaporative light-scattering detection

作　　者：吴昭晖 [1] 游文玮 [2] HE Feng  

机构地区：[1]南方医科大学分析测试中心,广东,广州,510515 [2]南方医科大学化学教研室,广东,广州,510515

出　　处：《第一军医大学学报》2005年第12期1549-1551,共3页Journal of First Military Medical University

摘　　要：目的建立高效液相色谱法-蒸发光散射检测器(HPLC-ELSD)测定苦杏仁中苦杏仁苷含量的的分析方法。方法采用Hypersil-ODS(4.0mm×250mm,5μm)色谱柱;流动相为甲醇-水(30∶70),流速0.5ml/min;柱温25℃;利用ELSD检测器检测:漂移管温度98℃,载气流速3.2L/min。结果苦杏仁苷进样量在1.0￣15.1μg范围内,标准曲线呈良好的线性关系,r=0.9999,苦杏仁苷的平均回收率为99.0%,RSD为2.9%(n=5)。结论本方法简便快速,结果准确可靠,为苦杏仁苷的含量测定提供了一个新方法,可用于苦杏仁药材及含苦杏仁复方制剂的质量控制。High-performance liquid chromatography with evaporative light-scattering detection （HPLC-ELSD） was employed for determination of amygdalin content in Semen Armeniacae Amarum. The detection was performed with the column of Hypersil-ODS （4.6 mm×250 ram, 5μm） and column temperature of 25 ℃. The mobile phase was methanol-water （70：30） with flow rate of 0.5 ml/min. Evaporative light-scattering detector was used and the drift tube temperature was set at 98℃ with the gas flow rate of 3.2 L/min. A standard curve was generated, which was linear in the range of 1.0-15.1 μg for amygdalin content （r=0.999 9）. The average recovery of amygdalin was 99.0% with RSD of 2.9% （n=5）. Besides simplicity and rapidness, the method yields accurate and reproducible results and can therefore be used in the quality control of Semen Armeniacae Amarum.

关 键 词：高效液相色谱法 蒸发光散射检测器 苦杏仁 苦杏仁苷 

分 类 号：R284[医药卫生—中药学]