热休克蛋白70对离体心脏心肌间质的保护作用  

The Effect of Heat Shock Protein 70 on Myocardial Interstitium of Isolated Rat Hearts

作　　者：孙忠东[1] 池一凡[1] 夏家红[2] 杨铁南[1] 侯文明[1] 

机构地区：[1]青岛大学医学院附属青岛市立医院,山东青岛266011 [2]华中科技大学同济医学院附属协和医院

出　　处：《中国微循环》2005年第6期400-402,共3页Journal of Chinese Microcirculation

基　　金：青岛市科委资助项目(KZD04-25)

摘　　要：目的探讨热休克蛋白70(HSP70)对大鼠离体心脏心肌间质的影响。方法W istar大鼠16只,分为2组:对照组(C,n=8),腹腔注射生理盐水0.4 m l,24 h后取离体心脏灌注HTK心脏保护液,4℃保存3 h后建立Langendorff灌注模型,灌注KH液2 h;实验组(E,n=8),腹腔注射重酒石酸去甲肾上腺素,24 h后取离体心脏,方法同对照组。以心肌细胞中HSP70含量、血流动力学指标、心肌组织羟脯氨酸(HP)、内皮素(ET)含量和心肌超微结构等作为观察指标。结果HSP70含量E组与C组比较明显增高;E组心功能恢复方面优于C组(P<0.05),HP含量优于C组(P<0.01),ET含量低于C组(P<0.01),心肌超微结构损伤较C组明显减轻。结论HSP70对供心心肌间质具有保护作用。Objective To investigate the protective effects of heat shock protein 70（HSP70） on myocardial interstitium of isolated rat heart. Methods 16 Wistar rats were randomly divided into 2 groups. In controll group （ C, n = 8）, distilled water was injected intraperitioneally and 24 hours later isolated hearts were perfused by Langendorff model and stored in 4℃ for 3 hours with Histidine - trytophan - ketoglurate（HTK） so- lutions, and then isolated hearts Langendorff model was perfused for 2 hours. In experimental group（ E, n = 8）, norepinephrine was injected intraperitioneally and 24 hours later isolated hearts were perfused by Langendorff model and stored in 4℃ for 3 hours with HTK solution, and then isolated hearts Langendorff model was per- fused for 2 hours. HSP70 content, the recovery of hemodynamics, hydroxyproline （HP） and endothelin （ET） content, and uhrastructure were tested. Results There was significant increase in HSP70, hemodynamics recovery and HP content in group E than group C, and significant decrease in ET in group E than in group C. Uhrastructure injury in group E is less than that in group C. Conclusion This study demonstrated that HSP70 has significant protective effect on myocardial interstitium of isolated rat heart.

关 键 词：热休克蛋白70 心肌间质 心肌保护 

分 类 号：R654.2[医药卫生—外科学] Q51[医药卫生—临床医学]