用旋转生物反应器体外再造骨组织的实验研究  

Experimental Research of Tissue Engineered Bone Formation in Vitro in a Spinner Flask Bioreactor System

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作  者:程晓兵[1] 陈富林[1] 毛天球[1] 胡晓光[1] 杨耀武[1] 侯锐[1] 孙沫逸[1] 

机构地区:[1]第四军医大学口腔医学院颌面外科,陕西西安710032

出  处:《口腔医学研究》2005年第6期638-640,共3页Journal of Oral Science Research

摘  要:目的:将骨髓基质干细胞接种于聚乙醇酸支架中,在旋转生物反应器内进行动态培养,观察骨组织的形成情况。方法:分离、培养、扩增猪骨髓基质干细胞,细胞长满后用地塞米松诱导,收集细胞,按5×107/mL的浓度50μL接种于聚乙醇酸支架中,静态培养5 d后,移入旋转生物反应器内进行动态培养,2月取材,通过大体标本、组织学检查观察新骨的形成情况,以静态培养作为对照。结果:细胞接种后1 d,即可以贴附于材料表面,显微镜下观察,细胞在材料表面伸展良好。连续培养2个月,形成质地坚硬的组织;组织学检查可见新形成的骨样组织,厚度约100μm,而对照组中在材料表面有纤维组织形成。结论:旋转生物反应器内进行动态培养是体外再造骨组织的有效手段。Objective: To evaluate in vitro new bone formation by bone marrow mesenchymal stem cells (MSCs) seeded into polyglycolic acid (PGA) scaffold in a spinner flask bioreactor system. Methods: Bone marrow mesenchymal stem cells (MSCs) were cultured, expanded and induced in vitro. Cells were seeded into PGA non -woven mesh at the density of 5 × 10^7/ml in 50μl cell suspension. Cell growth on the scaffold was observed by phase - contrast microscope. After 5 days static incubation, four cell -polymer complexes were transferred into a spinner flask bioreactor. Two months later, bone formation was observed by gross inspection examination and histological observation. Four cell -polymer complexes were incubated in static condition acting as control. Results: MSCs adhered and spreaded well on the surface of PGA scaffold. Hard tissue was restored successfully in the bioreactor 2 months after implantation. And histological examination demonstrated that newly restored tissue was composed of osteoid. In the control group only soft tissue formed on the surface of the scaffold. Conclusion: It was an efficient method to form new bone in vitro by seeded MSCs into PGA scaffold and incubated in a spinner flask bioreactor system.

关 键 词:组织工程 骨髓基质干细胞 聚乙醇酸 生物反应器 

分 类 号:R318.0[医药卫生—生物医学工程]

 

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