甲氨蝶呤囊泡的制备和体外释放性能研究  

Preparation of methotrexate niosomes and release of methotrexate from the niosomes in vitro

作　　者：杜美菊[1] 凌翠霞[1] 李娜[2] 赵凤林[2] 李克安[2] 

机构地区：[1]商丘师范学院化学系,河南商丘476000 [2]北京大学化学与分子工程学院,北京100871

出　　处：《应用化工》2005年第12期757-759,共3页Applied Chemical Industry

摘　　要：用非离子表面活性剂和胆固醇为原料,采用薄膜分散法制备甲氨蝶呤囊泡,通过透析法分离未包封的药物,紫外分光光度法测定包封率和体外释药性。结果显示,Span 60与胆固醇形成的囊泡(VSpan 60∶VCHOL=5∶3),在50℃超声40 m in的条件下,对0.60 g/L的甲氨蝶呤包封率可达65%以上,在模拟胃流体和模拟肠流体中均有良好的缓释作用。The methotrexate niosomes was prepared by non-ionic surfactant and cholesterol by aqueous dispersion of film. The free methotrexate was removed by dialysis. Entrapment efficiency and release of methotrexate from niosomes in vitro were measured by ultraviolet spectrophotometry. The results show that the entrapment efficiency of the niosomes prepared by span 60 and cholesterol （ VSpan 60：VCHOL= 5 ： 3 ） is more than 65% for methotrexate. The release profiles in simulated gastric and intestinal fluid showed a controlled release tendency to some extent.

关 键 词：甲氨蝶呤 非离子表面活性剂 囊泡 制备 缓释性 

分 类 号：R944.150.55[医药卫生—药剂学]