葛根芩连汤饮片汤剂、配方颗粒汤剂中黄芩苷含量比较  

Comparison of baicalin between slice decoction and dispensing granule decoction of Gegenqinlian prescription

作　　者：雷鹏[1] 刘韶[1] 李新中[1] 肖菁[1] 

机构地区：[1]中南大学湘雅医院,湖南长沙410008

出　　处：《中国医院药学杂志》2005年第11期1008-1010,共3页Chinese Journal of Hospital Pharmacy

基　　金：湖南省卫生厅中医药科研基金(编号:221201)

摘　　要：目的:比较葛根芩连汤饮片汤剂、配方颗粒汤剂中黄芩苷含量。方法:用高效液相色谱法测定葛根芩连汤中黄芩苷含量。色谱柱:Diamonsil C18(4.6mm×250mm,5μm);流动相:甲醇-0.05%磷酸(65∶35);流速:1mL.min-1;柱温:30℃;检测波长:280nm。结果:配方颗粒汤剂中黄芩苷含量为14.69mg.g-1;饮片汤剂中黄芩苷含量为10.45mg.g-1。结论:葛根芩连汤配方颗粒汤剂与饮片汤剂的色谱图基本一致,配方颗粒汤剂中黄芩苷含量均比饮片汤剂高。OBJECTIVE To compare the content of baicalin between slice decoction and dispensing granule decoction of Gegenqinlian. METHODS A HPLC method detected baicalin with 280 nm. Diamonsil C18 （4. 6 mm × 250 mm, 5μm） column was used, the mobile phase was a mixed solution of MeOH-0. 05% H3 PO4 （65：35）. The column temperature was setup at 30℃ and the flow rate was 1 mL·min^-1. RESULTS The content of baicalin in the dispensing granule decoction was 14. 69 mg·g^-1 ,and in the slice decoction it was 10. 45 mg·g^-l. CONCLUSION The HPLC chromatograms are similar in two forms of Gegenqinlian decoction. The content of baicalin of dispensing granule decoction is higher than that of slice decoction.

关 键 词：高效液相色谱法 葛根芩连汤 饮片汤剂 配方颗粒汤剂 黄芩苷 

分 类 号：R927.2[医药卫生—药学]