扶正抑癌方对人结肠癌细胞裸鼠肝转移的影响  

Effects of Fuzheng Yi'ai Decoction on Human Colon Cancer Liver Metastasis in Nude Mice

作　　者：盛学仕[1] 张瑞芳[2] 庞慧芳[1] 章忱[1] 卫洪昌[1] 

机构地区：[1]上海中医药大学基础医学院,上海201203 [2]上海市徐汇区中心医院,上海200031

出　　处：《上海中医药大学学报》2005年第4期39-41,共3页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：上海市科学技术发展基金资助项目(014019068)

摘　　要：目的观察扶正抑癌方抗大肠癌肝转移的作用,并探讨其可能的作用机制。方法将40只BALB/C裸鼠随机分为5组:模型组、中药组、化疗组、防治组、联合组,以5×106个/m l浓度的LoVo细胞悬液0.2 m l接种于裸鼠脾脏,建立大肠癌肝转移模型。后4组分别予扶正抑癌方治疗、5-Fu治疗、扶正抑癌方防治、扶正抑癌方+5-Fu治疗,分别从移植瘤和转移瘤的重量、形态学以及血清癌胚抗原浓度来观察扶正抑癌方的疗效。结果与模型组相比,防治组、联合组、化疗组脾脏接种瘤、肝脏转移瘤重量均减轻(P<0.05),其中联合组低于化疗组(P<0.05);而且联合组、化疗组CEA含量明显降低(P<0.05)。中药组上述指标无显著差异(P>0.05)。结论扶正抑癌方联合5-Fu可以降低模型动物的肝转移发生率,抑制脾脏接种瘤、肝脏转移瘤的生长。Objective To investigate the effects of Fuzheng Yi＇ ai Decoction on human colorectal cancer liver metastasis and to explore its mechanism. Methods Xenotransplanted human colon cancer cell line through intraspleen injection of 5 × 10^6/ml LoVo cells, models of liver metastasis of colorectal cancer were established on 40 BALB/C nude mice. The mice were randomized into 5 groups ： modle group （ MG）, Fuzheng Yi＇ ai Decoction treatment group （ FTG）, chemotherapy group （CTG） , prevention and cure group （ PCG）, and Fuzheng Yi＇ ai Decoction combined with 5-FU treatment group （FCTG）. Weight of inoculated tumor in spleen and metastasisic tumor in liver were measured, and the concentration of CEA in serum was detected by ELISA. Results Compared with MG, the weight of inoculated tumor and metastasisic tumor of PCG, FCFG, CTG decreased significantly （P 〈0.05）, and the weight of FCTG was lighter than that of CTG; CEA of CTG and FCTG decreased significantly （P 〈 0.05 ）. No effectiveness had been revealed in the FTG. Conclusion Fuzheng Yi＇ ai Decoction combined with 5-FU can decrease the ratio of liver metastases and inhibit the growth of the inoculated tumor liver metastases.

关 键 词：扶正抑癌方 大肠癌 转移 动物实验 

分 类 号：R273[医药卫生—中西医结合]