δ阿片受体激活对培养心肌细胞生长的影响  

Effects of δ-opioid receptor stimulation on proliferation of cultured myocardial cells

作　　者：包品[1] 王洪新[1] 梁科研[1] 王辉[1] 

机构地区：[1]锦州医学院药理学教研室,辽宁锦州121001

出　　处：《中国药理学与毒理学杂志》2005年第6期419-423,共5页Chinese Journal of Pharmacology and Toxicology

基　　金：辽宁省自然科学基金(20042170)~~

摘　　要：目的观测δ阿片受体激活对培养的心肌细胞是否有直接的促生长作用.方法体外培养乳大鼠心肌细胞.结晶紫法和[3H]TdR法测定心肌细胞的增生;流式细胞仪测定细胞周期百分比;Lowry等法测定培养细胞蛋白质含量;倒置显微镜下计数心肌细胞搏动频率.结果δ阿片受体激动剂D-丙(2)-D-亮(5)脑啡肽(DADLE)10 nmol·L-1～10 μmol·L-1能浓度依赖性促进细胞增殖,增加细胞蛋白质含量, 增加细胞搏动频率;高选择性δ阿片受体拮抗剂纳曲吲哚(naltrindole) 10 μmol·L-1对DADLE的促进作用有一定的抑制作用.结论δ阿片受体激活对体外培养的心肌细胞生长有促进作用.AIM To study if δ-0pioid receptor activation has direct effect on cultured cardiomyocyte growth. METHODS Myocardial cells of neonatal rats were cultured in vitro. The cellular proliferation was determined by crystal violet uptake and [^3H] TdR incorporation; the percentage of S ＋ G2 ＋ M in cell cycle was determined by flow cytometry; the protein content was assayed with Lowry, et al method ;contracting frequency of cultured myocardial cells was counted under the inverted microscope. RESULTS A 3-0pioid receptor agonist [ D-Ala^2, D-Leu^5 ]-enkephalin （DADLE） at 10 rlmol·L^- 1 _ 10μmol·L6- 1 concentration-dependently promoted the myocardial cells proliferation, increased protein content in culture cells and contracting frequency of the myocardial cells. Highly selective δ-opioid receptor antagonist naltrindole at 10 μmol·L^-1 inhibited the promoting effects of DADLE. CONCLUSION δ-Opioid receptor activation can promote growth in cultured myocardial cells.

关 键 词：受体 阿片样 δ 心肌 细胞 培养的 脑啡肽 亮氨酸-2-丙氨酸 纳曲吲哚 细胞增殖 

分 类 号：R96[医药卫生—药理学]