西藏砂生槐生物碱抑菌及杀虫活性的测定  

The Determination of Bacteriostasis and Insecticidal Activity of Alkaloids from Sophora moorcroftiana Seeds

作　　者：马兴铭[1] 李红玉[1] 王波[2] 尹少甫[1] 

机构地区：[1]兰州大学生命科学学院,兰州730000 [2]甘肃省康复中心

出　　处：《中国生物防治》2005年第3期183-186,共4页Chinese Journal of Biological Control

基　　金：甘肃省中青年自然科学基金项目(YS-011-A25-003);甘肃省中医管理局基金项目(2003-G2K-17)

摘　　要：用MTT比色法、抑菌圈法对西藏高原植物砂生槐生物碱进行了室内离体抑菌活性测定。结果表明,其对水稻白叶枯病菌KS-6-6J、XOⅢ、PXO99,以及水稻条斑病菌RS105、丁香假单胞菌PPB2038、马铃薯软腐病菌E的最低抑菌浓度分别达10.53、7.20、15.79、15.79、5.30、30.77mg/ml;对核果假尾孢、茄匍柄霉、褐孢霉的EC50值分别为12.90、15.79、12.55mg/ml。采用小叶碟添加法测定了对菜青虫的毒杀作用,其LD50值为326.59mg/L。The bacteriostasis activity of alkaloids from seeds of Sophora moorcroftiana growing in Tibet altiplano, were tested with MTT assay and bacteriostasis circle method in vitro. Results showed that minimum inhibitory concentration （MIC） of alkaloids on Xanthomonas oryzae pv. oryzae KS-6-6, JXOⅢ, PXO99, and X. oryzae pv. oryzicola RS105, Pseudomonas syringae pv. syringae PPB2038 and Erwinia carotovora pv. carotovora E were 10.53, 7.20, 15.79, 15.79, 5.30 and 30.77mg/ml, respectively, and the EC50 value on Pseudocercospora circumscissa, Stemphylium solani and Fulvia fulva were 12.90, 15.79 and 12.55mg/ml, respectively. Insecticidal activity of alkaloids to Pieris rapae was observed by antifeeding and LD50 was 326.59mg/L.

关 键 词：砂生槐 生物碱 抑菌作用 菜青虫 

分 类 号：S482.292[农业科学—农药学]