对生玉米离体培养再生体系的建立  

Construction of the Regeneration System for In Vitro Culture of Oppositifolious Maize

作　　者：崔广荣[1] 刘正[1] 张子学[1] 张从宇[1] 

机构地区：[1]安徽科技学院植物科学系,安徽凤阳233100

出　　处：《西北植物学报》2005年第12期2413-2419,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：安徽省自然科学基金项目(01041113);安徽科技学院自然科学基金项目(ZRC200212)

摘　　要：以对生玉米的雌、雄幼穗为外植体，研究了不同质量浓度激素及其组合对愈伤组织诱导、再分化苗和试管苗生根的影响，建立了对生玉米离体培养再生体系。结果表明：长度为15-17 mm的雌、雄幼穗能够诱导出质量较好的愈伤组织，但只有来自于雄幼穗的愈伤组织才能再生成苗。适合愈伤组织诱导的培养基为Ms+1．5-2．0 mg·L-12，4-D+0．5 mg·L-16-BA+0．5 mg·L-1 NAA+500 mg·L-1脯氨酸+1000 mg·L-1水解酪蛋白+30 g·L-1蔗糖；适合愈伤组织再分化培养基为MS+1．5-2．0 mg·L-1 6-BA+0．1 mg·L-1 NAA+500 mg·L-1水解酪蛋白+30 g·L-1 蔗糖；适合试管苗生根培养基为1／2 MS+0．25-0．5 mg·L-1 IBA+20 g·L-1蔗糖。Young tassels and ears of oppositifolious maize were used as the explants to study the effects of different hormones at different mass concentrations and their combinations on callus induction,shoot regeneration and shoot rooting and the regeneration system for in vitro culture of oppositifolious maize was established. The results showed that 15-19 mm long young tassels and ears could be induced to form quality calluses but only the calluses from the tassels could developed into the seedlings. The optimal medium for callus induction was MS＋1. 5-2.0mg·L^-1 2,4-D＋0.5 mg· L-1 6-BA＋0.5 mg· L^-1 NAA＋500 mg·L-1 proline ＋ 1 000 mg·L^-1 CH＋ 30 g ·L^-1 sucrose,the optimal medium for callus differentiation was MS＋1. 5-2. 0 mg·L^-1 6-BA＋0. 1 mg·L^-1 NAA＋500 mg·L^-1 CH＋30 g ·L^-1 sucrose,and the optimal for shoot rooting was 1/2 MS＋0.25-0. 5 mg·L^-1 IBA＋20 g ·L^-1 sucrose.

关 键 词：对生玉米 离体培养 再生体系 

分 类 号：Q813.1[生物学—生物工程]