秦川牛及其杂种牛POU1F1基因多态与生长性能相关性  

Correlation of Polymorphisms of POU1F1 Gene and Growth Traits in Qinchuan Cattle and Its Hybrid Cattle

作　　者：刘波[1] 陈宏[1] 蓝贤勇[1] 雷初朝[1] 张志清[1] 张润锋[1] 

机构地区：[1]西北农林科技大学动物科技学院/陕西省农业分子生物学重点实验室,杨凌712100

出　　处：《中国农业科学》2005年第12期2520-2525,共6页Scientia Agricultura Sinica

基　　金：国家"863"计划项目(2003AA243051);国家自然科学基金(30471238);西北农林科技大学拔尖人才基金资助

摘　　要：本研究首次利用PCR-RFLP技术研究相同季节4月(±10d)龄纯种秦川牛(QQ)及杂种牛秦安(AQ)、秦德(DQ)、秦利(LQ)4个群体164个个体POU1F1基因多态性及其与体重、体尺等生长性状指标之间的相关性。结果表明,QQ及AQ、DQ、LQ群体POU1F1-HinfI基因座的451bp的PCR产物被限制性酶HinfI消化后表现多态性,它们的等位基因A/B频率分别为:0.232/0.768、0.333/0.667、0.178/0.822和0.181/0.819,且均处于Hardy-Weinberg平衡状态。同时,4个群体POU1F1基因座不同基因型与体重、体尺等生长性状指标相关分析的结果表明,4个群体内AB、BB基因型个体在胸围、十字部高指标上显著高于群体AA型个体(P<0.05),即BB、AB>AA(P<0.05),可作为秦川牛体尺指标(胸围、十字部高)候选基因之一。但在体长指标上均无显著差异(P>0.05),所以不宜作为体长指标候选基因。初步认为BB基因型为优势基因型,相应地B为优势等位基因,对选择有正向效应。PCR-RFLP was firstly applied to analyze the correlation of polymorphisms of POUIFI gene and growth traits in 164 beef cattle, at the age of 4 months （±10 d）, including Qinchuan cattle, QinchuanxLimousin cattle, QinchuanxRed Angus and QinchuanxGerman Yellow cattle. The results showed that 451 bp PCR products digested with Hinfl demonstrated polymorphisms in four populations which were at Hardy-Weinberg equilibrium （P〉0.05）. Moreover, the frequencies of allele A/B of QQ, AQ, DQ and LQ populations were 0.232/0.768, 0.333/0.667, 0.178/0.822 and 0.181/0.819, respectively. The correlation of polymorphisms of POUIFI gene and growth traits among the 4 populations was analyzed. In heart girth, height at hip cross, individuals with genotype AB and BB in population were higher than that of genotype AA, that is, BB and AB 〉 AA （P〈 0.05）. So POU1F1 gene can be one of a candidate genes for growth traits with heart girth, height at hip cross, but not for body length trait. The conclusion is that genotype BB is a predominant genotype and B is a predominant allele.

关 键 词：秦川牛 PCR—RFLP POU1F1基因 多态性 生长性能 

分 类 号：S823.3[农业科学—畜牧学]