猪SLA-DQA一个新SNP的发现及其遗传效应的研究  

Characterization of a Novel SNP of SLA-DQA and Its Effects on Carcass Traits

作　　者：彭勇波[1] 李奎[2] 樊斌[1] 余梅[1] 朱猛进[1] 熊统安[1] 彭中镇[1] 刘榜[1] 

机构地区：[1]华中农业大学动物科技学院动物分子生物学与育种实验室,武汉430070 [2]中国农业科学院畜牧研究所,北京100094

出　　处：《中国农业科学》2005年第12期2526-2530,共5页Scientia Agricultura Sinica

基　　金：国家自然科学基金(30371029);湖北省科技攻关项目(2005AA206B03);国家"863"计划(2004AA222170);教育部重点项目(01121);学校创新基金项目资助

摘　　要：采用PCR-RFLP方法对新发现的一引起氨基酸改变的SNP位点(读码框第722位碱基)进行了检测,初步探讨了DQA基因作为影响经济性状候选基因的可能性。经在6个中国地方猪种及大白猪中PCR-RFLP检测,发现除小梅山和大白猪外,其它几个猪种均存在多态性,同时对6个地方猪种间的基因型频率的差异进行了χ2检验。在通城猪、长白猪、大白猪及其三元杂种猪长大通和达长通猪中检测了该位点的基因型,并运用GLM进行基因型与生长、胴体性状间关联分析,发现该SNP位点与平均背膘厚、6-7肋间背膘厚存在显著相关(P<0.05),与胸腰椎间背膘厚也接近显著性水平(P=0.06),与内脂率、眼肌高、肌肉失水率亦存在显著相关(P<0.05),但未发现与生长性状间的相关。A novel SNP at the nucleotide 722 of coding sequence, which caused shift mutation, were detected using PCR-RFLP. This study was designed to investigate the effect of DQA on pig economic traits. The results of genotypes in six Chinese indigenous breeds and Large White showed that there exist polymorphism excepting Xiaomeishan Pigs and Large White. The differences in genotype frequency were scored using the x^2 value among the 6 indigenous breeds. The genotype distributions were also analyzed in Large White, Landrace, Tongcheng, Landrace×（Large WhitexTongcheng） and Large Whitex（LandracexTongcheng）, and the association analysis between genotype and traits was performed by GLM. The results showed that the genotypes have significant correlations with average backfat thickness, backfat thickness between 6^th and 7^th ribs, percentage of leaf and caul and eye muscle width （P〈0.05）, and nearly significant correlation levels with backfat at the loin （P=0.06）. However, there were no significant associations between DQA genotype and growth traits.

关 键 词：猪 SLA—DQA 第四外显 SNP PCR—RFLP 关联分析 多态性 

分 类 号：S828.3[农业科学—畜牧学]