益气活血法对肺纤维化动物模型细胞因子表达的影响  

Effects of Yiqihuoxue on cytokine expression in pulmonary fibrosis model in rats

作　　者：张欣[1] 辛洪涛[1] 于静[2] 李怀臣[1] 白雪丽[1] 黄琛[1] 宋秀杰[3] 

机构地区：[1]山东省立医院呼吸内科,山东济南250021 [2]济南第二人民医院急诊科,山东济南250021 [3]北京海军总医院呼吸内科,北京100037

出　　处：《山东大学学报（医学版）》2005年第12期1128-1131,共4页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助课题(30271625)

摘　　要：目的:探讨益气活血颗粒剂对纤维化肺组织TGF-β1、TNF-α表达的影响。方法:20只Wistar雄性大鼠随机分为模型组、中药治疗组、激素组及正常对照组4组,采用BLEMA5建立大鼠肺间质纤维化模型,中药治疗组给予益气活血颗粒剂(250mg/kg)灌胃,激素组应用氢化考的松(25mg/kg)肌注。观察肺组织HE、VG染色病理改变,采用核酸原位杂交法检测TGF-β1、TNF-α表达量。结果:模型组肺泡炎和肺纤维化程度较重,且其胶原纤维沉积较多,TGF-β1、TNF-α的表达增多;而中药治疗组肺泡炎和纤维化程度较轻,胶原沉积明显减少,TGF-β1、TNF-α表达减少。激素组纤维化程度比模型组减轻,但比中药组重,TGF-β1、TNF-α表达减少不明显。结论:益气活血颗粒剂通过下调TGF-β1、TNF-α的表达而明显减轻了肺间质纤维化程度。Objective：To investigate the effect of Yiqihuoxue （YQHX） on TGF-β1 and TNF-αexpressions in lung tissues in bleomycin-induced pulmonary fibrosis model in rats. Methods： Animal model of bleomycinA5-induced pulmonary fibrosis was established by BLEMA5 in rats. 20 Wistar rats were divided into normal control, fibrosis, treatment and corticosteroids groups. Normal control group was given 0.9% saline （2 ml/kg） intratracheally by instillation, fibrosis group bleomycinA5（5 mg/kg）intratracheally by instillation, treatment group YQHX 1,2,3 （250 mg/kg） daily by oral , and corticosteroids group hydrocortisone （25 mg/kg im） daily. All rats were killed on 28 days. The HE and the VG stain were pathologically examined and the expression of TGF-β1 and TNF-α mRNA in the lung tissues were analyzed by nucleic acid hybridization in situ. Results：YQHX1,2,3 obviously alleviated rats alveolitis and fibrosis. TGF-β1 and TNF-α levels were higher in fibrosis group than those in normal control and treatment group （P〈0.01）. Conelusion：YQHX1,2,3 can treat pulmonary fibrosis through reducing the expression of TGF-β1 and TNF-α in lung tissues.

关 键 词：肺纤维化 益气活血 转化生长因子β 肿瘤坏死因子α 大鼠 WISTAR 动物模型 

分 类 号：R563[医药卫生—呼吸系统]