结晶紫线性扫描伏安法测定肝素钠的研究  

Linear Sweep Voltammetric Determination of Heparin Sodium with Crystal Violet

作　　者：孙伟[1] 丁雅勤[1] 韩军英[1] 焦奎[1] 怀盈盈[1] 

机构地区：[1]青岛科技大学化学分子与工程学院,青岛266042

出　　处：《药物分析杂志》2005年第12期1495-1498,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：中国博士后科学基金(2003033492);国家自然科学基金(20405008;20375020);青岛市自然科学基金(04-2-JZ-114)

摘　　要：目的:以结晶紫为探针建立了一种电化学分析测定肝素钠的新方法。方法:在 pH 3.0的 Britton-Robinson(B-R)缓冲溶液中,结晶紫在-0.72 V(vs.SEE)处有一个灵敏的线性扫描二阶导数极谱波,当加入一定量的肝素钠后,两者之间通过静电引力会形成一种生物超分子复合物,导致溶液中游离的结晶紫的浓度降低,相应的还原峰电流降低,进而可以用于肝素钠的测定。结果:优化了结合反应条件和电化学测定条件,在最佳实验条件下,峰电流的降低值与肝素钠的浓度在0.1～8.0 mg·L^(-1)范围内呈线性关系,线性回归方程为△i_p″(nA)=400.4+1563.1C(mg·L^(-1))(n=14,r=0.993)。结论:将该方法应用于肝素钠注射液测定,结果令人满意。Objective： A new electrochemical method for the determination of heparin sodium was established based on the binding reaction of heparin sodium with crystal violet. Methods： In pH 3.0 Britton - Robinson （ B - R） buffer solution,crystal violet （CV） has a well -defined second order derivative linear sweep vohammetric peak at0. 72 V （vs. SCE）. On the addition of heparin sodium into the CV solution, the reductive peak current of CV decreases without the movement of peak potential and the appearances of new peaks, which is due to the interaction of CV with heparin sodium to form a new supramolecular complex. The free concentration of CV in solution was decreased, so the decrease of peak current of CV, which can be used for the determination of heparin sodium. Results： The conditions of interaction and electrochemical determination were optimized. Under the optimal conditions the decrease of reductive peak current is in proportional to the heparin sodium concentration in the range of 0. 1 - 8.0 mg · L^-1 with the linear regression equation as △ip^n（nA） = 400. 4 ＋ 1563.1 C（ mg · L^-1 ） （ n = 14, r = 0. 993 ）. The effects of coexisting substances on the determination of heparin sodium were investigated and the results showed that this method had good selectivity. Conclusion：This new electrochemical method was further applied to the determination of the heparin sodium injection samples with satisfactory results.

关 键 词：肝素钠 结晶紫 极谱分析 结合反应 线性扫描伏安法 

分 类 号：R927[医药卫生—药学] R972.4