RP-HPLC测定黄珠子草中短叶苏木酚的含量  

RP-HPLC Determination of Brevifolin in Phyllanthus simplex

作　　者：牛晓峰[1] 贺浪冲[1] 徐东伟[1] 

机构地区：[1]西安交通大学医学院药学系,西安710061

出　　处：《药物分析杂志》2005年第12期1524-1526,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：陕西省自然科学基金(No.2001SM72)资助

摘　　要：目的:建立高效液相色谱法测定黄珠子草中短叶苏木酚的含量。方法:以 Thermo Hypersil-Keystone ODS C_(18)为分析柱,甲醇-1%醋酸(36:64)为流动相,流速1.0mL·min^(-1),检测波长278nm,采用外标法定量测定。结果:短叶苏木酚在10～100μg·mL^(-1)范围内线性关系良好(r=0.999 7);其平均回收率为98.77%,RSD 为0.98%(n=6)。结论:该方法简便可靠,快速,重现性好,可用于黄珠子草中短叶苏木酚的含量测定。Objective： To establish an HPLC method for the determination of brevifolin in Phyllanthus simplex. Method：RP-HPLC analysis was carried out on Thermo Hypersil-Keystone ODS C18 column and the mixture of Methanol- 1% HAc （36： 64） was used as the mobile phase ,the detection wavelength at 278 nm,and the flow rate at 1.0 mL · min^-1. Result：The standard curve was linear over the range of 10 ～ 100μg · mL^-1 （r = 0. 999 7 ）, and the average recovery was 98.77 % with RSD 0. 98%. Conclusion：The method is sensitive, accurate and specific. It may be used for the quantitative determination of brevifolin in Phyllanthus simplex.

关 键 词：黄珠子草 短叶苏木酚 反相高效液相色谱法 测定 

分 类 号：R282.71[医药卫生—中药学] R284.1[医药卫生—中医学]