RP-HPLC法研究塔斯品碱在大鼠体内的药代动力学  

Pharmacokinetics of Taspine in Rats by RP-HPLC

作　　者：李义平[1] 强科[1] 贺浪冲[1] 

机构地区：[1]西安交通大学医学院,西安710061

出　　处：《药物分析杂志》2005年第12期1534-1536,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：西安市重大技术创新项目(No.ZX05010)资助

摘　　要：目的:建立大鼠血浆中塔斯品碱浓度的 RP-HPLC 分析方法,并研究其药代动力学特性。方法:用液液萃取技术对血浆中的塔斯品碱进行纯化、浓集,用 RP-HPLC 法进行测定。色谱柱:Kromsil C_(18)ODS 柱(150mm×4.6mm,5μm);流动相:甲醇-60 mmol·L^(-1)磷酸二氢钠-20 mmol·L^(-1)SDS(70:30);流速:1.0 mL(min^(-1);检测波长:245nm;柱温:室温。结果:方法线性范围为15.63～903.7 ng·mL^(-1)(r=0.994 0),日内、日间精密度的 RSD 分别为3.8%～4.9%和4.2%～7.6%,平均回收率为(107.33±7.3)%～(97.30±4.8)%。塔斯品碱在大鼠体内的达峰时间约2.84 h,平均峰浓度为64.15 ng·mL^(-1),药时曲线下面积为1214.98 ng·mL^(-1)·h,消除半衰期为10.96 h。结论:分析方法灵敏、准确,适合于塔斯品碱的药代动力学研究。塔斯品碱经大鼠口服吸收较慢,而消除很慢。Objective：To establish an RP-HPLC method for the determination of taspine in rat blood plasma and to study its pharmacokinetics. Method：The blood plasma samples were isolated by liquid-liquid extraction and analyzed by the RP-HPLC method, the chromatographic conditions used in this study were of Kromsil C18 ODS column （150 mm 4. 6 mm,5 μm）as analytical column, methanol- 60 mmol · L^-1phosphate buffer- 20 mmol · L^-1 SDS （70： 30）as mobile phase and at the flow-rate of 1.0 mL · min^-1. The UV detection was set at 245 nm. Results：The calibration curve was linear in blood plasma over the concentration range of 15.63 ～ 903.7 ng · mL^-1 （ r = 0. 994 0）. Within-day and between-day precisions expressed as the relative standard deviation（RSD） were 3.8% ～ 4. 9% and 4. 2% ～ 7.6% respectively. Average recoveries added were（ 107.33 ± 7.3 ）% ～ （97.30 ± 4.8）%. The parameters were Tmax = 2. 84 h, Cmax = 64. 15 ng · mL^-1, AUC = 1214.98 ng · mL^-1 · h, T1/2（Ke） = 10. 96 h. Conclusion：The analytical method established in this study is stable and reliable, and can be used to measure the pharmacokinetics of taspine in rat. The experimental results show that taspine can be absorbed slowly, and slowly eliminated after oral administration.

关 键 词：塔斯品碱 高效液相色谱法 药代动力学 RP-HPLC法 

分 类 号：R969.1[医药卫生—药理学]