桃潜隐花叶类病毒中国分离株P_3的克隆与序列分析  

Cloning and nucleotide sequence analysis of PLMVd isolate P_3 from Chi na

作　　者：徐文兴[1] 王国平[1] 何云蔚[1] 洪霓[1] 

机构地区：[1]华中农业大学植物科技学院,武汉430070

出　　处：《植物病理学报》2005年第4期300-304,共5页Acta Phytopathologica Sinica

基　　金：科技部"八六三"课题资助项目(2001AA241142)

摘　　要：本研究以提取的类病毒cRNA为模板,采用RT-PCR方法,从5株表现明显花叶症状的'雨花露'桃树上检测到桃潜隐花叶类病毒(PLMVd),并对其中的样品P3通过2次RT-PCR扩增,然后分别将扩增产物回收、克隆测序,确定了该分离株(命名为PLMVd-P3分离株)的全长核苷酸序列,其cDNA分子全长为337 nt,这与已经报道的PLMVd典型分离株基因组大小相似.序列比较分析结果表明,该分离株与已经报道的不同地区来源的其它分离株相似性为90%～96%.Peach latent mosaic viroid （PLMVd） was detected from five plants of peach cultivar “Yuhualu” showing typical mosaic symptom by RT-PCR. RT-PCR product of P3samplewas collected and cloned into the vector pMD18-T. Full-length cDNA of the viroid was sequenced. The result showed the isolate had a size 337 nt, similar to that of typical PLMVd variants. Nucleotide sequence of the isolate had similarities of 90% - 96% with other reported isolates from different areas.

关 键 词：桃潜隐花叶类病毒 RT-PCR 克隆 序列 生物学特性 

分 类 号：S436.621[农业科学—农业昆虫与害虫防治]