一种新型微通道分析系统——发光二极管诱导荧光检测器  

A Novel Light-Emitting Diode-Induced Fluorescence Detector for Micro-Scale Flow Analysis System

作　　者：杨丙成[1] 谭峰[1] 关亚风[1] 

机构地区：[1]中国科学院大连化学物理研究所,辽宁大连116012

出　　处：《光谱学与光谱分析》2005年第12期1995-1997,共3页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金(20227501)资助项目

摘　　要：报道了一种新型发光二极管诱导荧光检测器。该系统采用共线性光学结构、通过柱上检测可与微 通道分析系统在线联用。以异硫氰酸荧光素(FITC)及其标记的氨基酸为标样、结合毛细管电泳和流动注射 技术评价了该系统。对FITC标记的苯丙氨酸浓度检测限为10-8 mol·L-1(柱上检测,0．05 mm内径),在 1×10-7-2×10-5mol·L-1有很好的线性关系(r2=0．999),6次连续进样所得峰高值相对标准偏差(RSD) 小于3％。A compact light-emitting diode-induced fluorescence detector based on collinear scheme is described. The detector was assembled by all-solid-state optical-electronic components and easily coupled with micro-scale flow analysis system by on-column detection mode. Fluorescein isothiocyanate （FITC） and FITC-labeled amino acids were used as test analytes to evaluate the detector performance by capillary electrophoresis and flow injection analysis techniques. The concentration limit of detection was 10^-8 mol·L^-1 for FITC-labeled phenylalanine at a signal-to-noise ratio （S/N） of 3 （on-column, 0.05 mm i. d. ）. The system exhibited a good linear response in the range of 1 ×10^-7-2 ×10^-5 mol·L^-1 （ r^2 = 0. 999）. The relative standard deviation （RSD） of 6 repetitive injections on peak heights is less than 3 %.

关 键 词：发光二极管 荧光 共线性 

分 类 号：O657.3[理学—分析化学]