超声诱导对家蝇幼虫基因组DNA影响的谱学研究  

Effects of Ultrasound Induction on Genomic DNA of Housefly Larvae

作　　者：安春菊[1] 李锋[2] 张立金[2] 李德森[1] 杜荣骞[1] 

机构地区：[1]南开大学生命科学学院,天津300071 [2]中国农业大学应用化学系,北京100094

出　　处：《光谱学与光谱分析》2005年第12期2020-2023,共4页Spectroscopy and Spectral Analysis

基　　金：天津市自然科学基金(03360491)资助项目

摘　　要：超声诱导3日龄家蝇幼虫后提取对照和处理组的基因组DNA,利用紫外光谱、荧光光谱、红外光 谱、核磁共振谱对其结构进行分析,并利用PCR对基因组中attacin基因的3’端序列进行测序比较。结果表 明,超声诱导能够影响家蝇幼虫基因组DNA的二级结构和碱基堆积状态,进而在DNA复制时造成碱基错 配最终导致DNA序列发生改变,但是超声诱导对DNA的化学基团和化学键没有明显的影响。The genomic DNA of housefly larvae was extracted after ultrasound induction, and the structure was analyzed by UV, fluorescence, IR and ^1H NMR. The 3′-end of attacin gene was sequenced and compared by means of PCR. All the results indicated that ultrasound induction can destroy the second structure and the base stacking of genornic DNA of housefly larvae, which will result in mismatch repair during DNA duplication and finally change the sequence of DNA, but it has no significant effect on chemical groups and chemical band of genomic DNA.

关 键 词：家蝇幼虫基因组DNA 超声诱导 DNA二级结构 

分 类 号：O657.3[理学—分析化学]