血液激发荧光强度分析及应用  

Analysis and Application of Excitation Fluorescence Intensity of Blood

作　　者：赵志敏[1] 郭林峰[1] 俞晓磊[1] 马军艳[2] 王乐新[1] 辛玉军[1] 

机构地区：[1]南京航空航天大学理学院,江苏南京210016 [2]青岛大学理工学院,山东青岛266071

出　　处：《光谱学与光谱分析》2005年第12期2056-2060,共5页Spectroscopy and Spectral Analysis

基　　金：南京航空航天大学创新基金项目资助

摘　　要：文章采用荧光分析技术,对血液激发荧光强度进行研究。给出了有关理论分析,并给出了正常血样 与异常血样(高血糖、高胆固醇)对比实验结果。研究发现:血液中血糖的浓度影响血液的激发荧光,其大致 趋势是:在相同波长的激发光激发下,随着血糖浓度的提高,血液的激发荧光强度也逐渐增强,显然,血液 中的血糖也是一种荧光物质,其浓度对血液的荧光强度的影响与理论分析相一致,这表明所得的实验结果 是合理的,同时还表明了可以通过比较血液的激发荧光强度来区分血液中血糖浓度的高低;研究还发现:胆 固醇含量越高,所得的荧光强度也越强。因此,根据血清的荧光强度可以知道血清中胆固醇含量的高低,尤 其是当使用波长位于435 nm附近的激发光时,结果非常明显。文章提出的血液激发荧光强度分析技术,为 血样快速检测及疾病诊断提供了新的途径。The analysis technique of fluorescence is adopted to study the intensity of excitation fluorescence of blood in the present paper. The theoretical analysis and differences of normal and abnormal blood （hyperglycenaia, hyperlipemia） are presented. The theoretical analysis was proved by experiment results. It was discovered that blood sugar consistency has an effect on blood fluorescence. In other words, with the same excitation wavelength and with blood sugar consistency increasing, the fluorescence intensity increases gradually. It is obvious that blood sugar is also a kind of fluorescein, and its consistency has an effect on fluorescence intensity, which is identical with the theoretical analysis, indicating that the experiment is successful, and it is possible to distinguish blood sugar consistency by comparing the fluorescence intensity in blood. It was also discovered that the higher the cholesterin content, the more intense the fluorescence, When excitation wavelength is especially 435 nm, the phenomenon is very obvious. The study paves a new way for the blood quick check and diagnosis of diseases.

关 键 词：血液 激发荧光 荧光强度 

分 类 号：O433.4[机械工程—光学工程]