悦肝胶囊对肝纤维化大鼠防治作用的实验研究  

The Protective Effects of Yuegan Capsule on Dimethylnitrosamine-induced Hepatic Fibrosis in Rats

作　　者：金政[1] 李佳[1] 李善花[1] 金美善[1] 

机构地区：[1]延边大学医学院组胚教研室,吉林延吉133000

出　　处：《辽宁中医杂志》2005年第12期1322-1324,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：吉林省科技攻关项目(200206)

摘　　要：目的:观察悦肝胶囊对二甲基亚硝胺(DMN)模型大鼠肝纤维化形成的影响,探讨悦肝胶囊抗肝纤维化的作用机制。方法:模型制备采用DMN4周12天腹腔注射,实验组分别用大、中、小剂量悦肝胶囊干预,干扰素对照。生化测定肝组织羟脯氨酸含量(HYP);免疫组织化学方法观察α-平滑肌肌动蛋白(α-SMA),基质金属蛋白酶-1(MMP-1)及其抑制剂(TIMP-1)的表达情况;通过天狼猩红染色和HE染色观察肝组织胶原沉积情况及病理变化。结果:与模型对照组相比,悦肝胶囊可有效改善肝组织胶原沉积,降低肝组织HYP含量,抑制肝组织α-SMA和TIMP-1表达,对MMP-1表达影响微弱,无差异。结论:提示悦肝胶囊具有保肝和抗肝纤维化作用,作用机制可能与其抑制肝星状细胞(HSC)活化,减少胶原合成,同时促进胶原分解有关。Objective：To investigate the effects and me-chanisms of Yuegan capsule on fiver fibrosis induced by dimethylnitrosamine （DMN） in rats. Methods： the animal models were made with 0.5% DMN ip. 3 days every week for 4 weeks. During the time, Yuegan capsule was administrated at the high dose, middle dose and small dose once a day compared with recombinant human interferon α-2a for injection. We detected and measured the content of in fiver homogenate, and observed alpha-smooth muscle actin （α-SMA）, matrix metalloproteinase-1 （MMP-1） and the tissue inhibitor of metalloprotei- nase-1 （TIMP-1） by immunohistochernical method. The fiver pathological changes and the deposition of collagen were observed with HE and Sirius red staining. P,.esults： compared with the model group, Yuegan capsule could decrease the deposition of collagen and the content of hydroxyproline efficiently in liver, and inhibit alphasmooth muscle actin （α- SMA） and the tissue inhibitor of metalloproteinase-1 （TIMP-1）. MMP-1 in all groups were slight and have no distinct dis-crepancy. Conclusions： Yuegan capsule is effective in protecting against DMN - induced liver fibrosis in rats. The mechanisms of the protection may due to in -hibit the activity of hepatic stellate cell （HSC）, then reduce the synthesis of the collagen and promote its degradation.

关 键 词：悦肝胶囊 肝纤维化 二甲基亚硝胺 基质金属蛋白酶抑制剂-1 实验研究 

分 类 号：R285.5[医药卫生—中药学]