小花盾叶薯蓣多倍体诱导及细胞学初步研究  

Polyploidy induction of Dioscorea parviflora and its cytological observation

作　　者：郑思乡[1] 吴福川[1] 谢世清[1] 李涵[1] 

机构地区：[1]云南农业大学,云南昆明650201

出　　处：《西南农业学报》2005年第6期822-824,共3页Southwest China Journal of Agricultural Sciences

基　　金：云南省自然科学基金项目(2003C0039M)

摘　　要：在离体培养条件下,比较了不同浓度,不同处理时间的秋水仙素对小花盾叶薯蓣丛生芽的诱变效果。结果表明:0.05%的秋水仙素处理24 h的诱变效果最佳,诱变频率高达52%。同时采用不定芽技术对变异株进行稳定,获得了稳定的多倍体。对变异株进行细胞学研究后发现,变异株的染色体数目为2n=4x=40,为四倍体;对照为二倍体,其染色体数目为2n=2x=20。另外还发现三倍体及非整倍体细胞的存在。Dioscorea parviflora was treated with colchicines at different concentration and time duration in vitro. The highest induction frequently （52 % ） was achieved with colchicines at 0.05 % for 24 h. Stable polyploid plants were obtained by the application of adventitious bud technique. Cytological observations confirmed the variant was a tetraploid with chromosome number of 2n = 4x = 40, while the cheek a diploid with chromosome number of 2n = 2x = 20. In addition, triploid and aneuploid cells were also observed in some variants .

关 键 词：小花盾叶薯蓣 多倍体 秋水仙素 染色体 

分 类 号：S531[农业科学—作物学]