中枢神经系损伤后不同时期星形胶质细胞的变化  

THE TIME-COURSE CHANGES OF ASTROCYTES FOLLOWING AN EXCITOTOXIC LESION IN THE CNS

作　　者：冯家笙[1] 徐慧君[1] 武义鸣[1] 田美玲[1] 

机构地区：[1]南通医学院神经生物学研究室

出　　处：《解剖学报》1996年第1期11-14,共4页Acta Anatomica Sinica

摘　　要：将神经毒６－羟多巴胺（６－ＯＨＤＡ）注入大鼠一侧中脑腹侧被盖区，用胶质原纤维酸性蛋白（ＧＦＡＰ）抗体对损伤鼠中脑切片进行免疫组织化学ＡＢＣ法检测，观察星形胶质细胞在受损伤１ｄ至３０ｄ的不同时期的变化。术后１～３ｄ，针道所经处见有ＧＦＡＰ阳性反应的细胞和纤维，其胞体增大，纤维短粗。术后１周，针道周围反应性胶质细胞增多，有些反应性胶质细胞发出长的突起横向延伸垂直于针道，损害区附近血管增生，紧贴血管外周的胶质细胞也出现反应性变化。术后１０～１４ｄ，损害区反应性胶质细胞进一步增多，与针道方向垂直排列的胶质细胞突起更多、更长，受损侧中脑脚底的胶质细胞胞体较对侧增大，突起也增粗。术后３０ｄ与１４ｄ时的变化类似，但垂直于针道的横向纤维已不如１４ｄ时明显。用等量生理盐水代替神经毒注射后，针道周围的胶质变化除胶质反应略轻外与神经毒组基本相似。本研究表明，中枢神经系受损伤时星形胶质细胞的反应性变化在１０～１４ｄ达到高峰，以后渐趋稳定。Neurotoxin 6-hydroxydopamine(6-OHDA) was injected into the ventral tegmentum of the mesencephalon in rats.The astrocytes were identified by using an antibody against glial fibrillary acidic protein(GFAP) and the progression of their reaction to the lesion was examined from 24 hours to one month after the lesion. One to three days after the lesion,there was an initial appearance of a number of GFAP positive cells along the needle track and these cells were enlarged with short and thick processes. One week after the lesion the number of reactive astrocytes increased,some of which extended out with long and straight processes perpendicular to the wounded track.The number of vessels in the lesion area were increased and the astrocytes adhering to the vessels enlarged compared with those showed in the contralateral side, Ten to fourteen days after the lesion the GFAP positive cells increased progressively and the long GFAP positive fibers perpendicular to the needle track became much more obvious, The labeled GFAP cell showed more active reaction in the ventral midbrain of the lesioned side than those of the contralateral side. One month after the lesion the glia changes seemed similar to those caused two weeks after the lesion, but the fibers perpendicular to the needle track were diminished. The glia changes in the animals injected with normal saline were slightly lighter than those in the excitotoxin-lesioned animals. The results showed that after CNS lesion the glial reaction in the vicinity of the lesion was most pronounced on the 10-14th day.

关 键 词：6-羟多巴胺 星形胶质细胞 中枢神经损伤 

分 类 号：R651.02[医药卫生—外科学]