Ghrelin对GH3细胞膜电位的作用  

Reduction of membrane potential in GH3 cells with ghrelin

作　　者：韩雪峰[1] 朱运龙[1] 陈晨[1] 

机构地区：[1]第四军医大学生理学教研室,陕西西安市710032

出　　处：《中国康复理论与实践》2005年第11期927-928,共2页Chinese Journal of Rehabilitation Theory and Practice

摘　　要：目的观察ghrelin对GH3细胞膜电位的作用。方法用Nystatin打孔全细胞记录跨膜电流,观察ghrelin组和对照组GH3细胞膜电位。结果对照组细胞的膜电位为-(72±4.78)mV,在ghrelin处理后,膜电位减小到(54.8±4.58)mV。结论ghrlein可以快速、可逆、显著性地降低GH3细胞膜电位。Objective To investigate the in vitro effect of ghrelin on membrane membrane potentail of GH3 cells. Methods Nystatin-perforated whole-cell recording configuration was used. Results The membrane potentail of GH3 was decreased from -（72 ± 4.78） mV to （54.8±4.58） mV（P〈0. 01） after application of gbrelin （10^-7 -10^-8 M）Conclusion Gbrelin can transiently and significantly decreased the amplitude of membrane potentail, which may contribute to the gbrelin-stimulated GH secretion from GH3 cells.

关 键 词：GHRELIN 膜片钳 膜电位 生长激素 

分 类 号：R338.2[医药卫生—人体生理学] R329.2[医药卫生—基础医学]