蔓生百部组织培养快繁技术  

Techniques for tissue culture and rapid propagation of Stemona japonica(Bl.) Miq

作　　者：李克烈[1] 林乙明 徐立[1] 李志英 马千全[1] 罗联忠[1] 黄碧兰[1] 陈伟[1] 

机构地区：[1]中国热带农业科学院作物品种资源研究所,海南儋州571737 [2]海南省东方市农业服务中心,海南东方572800

出　　处：《广西农业科学》2005年第6期505-506,共2页Guangxi Agricultural Sciences

基　　金：国家科技基础条件平台重点项目"热带作物种质资源收集;整理;保存和保护关键技术和标准研究"(编号:2003DIA3N022)

摘　　要：对蔓生百部的茎段上侧芽进行组织培养,结果表明:初代培养在M S+6 BA 2m g/L+kT 1m g/L+NAA0.2m g/L培养基上,约10d茎段上侧芽开始萌发;继代繁殖在M S+6 BA 2m g/L+NAA 0.2m g/L培养基上,约30d几乎全部诱导形成多芽体,多芽体的芽数平均为3个左右;生根壮苗一般采用1/2M S+IBA 0.2m g/L培养基。The axillary bud on the upper stem fragments of Stemona japonica （BI.） Miq were used as explants for tissue culture. The results showed that the axillary bud began to germinate about 10 days after primary culture on MS medium containing 2mg/L 6 -BA ＋1mg/L KT ＋0.2mg/L NAA, the clumpy buds were developed about 30 days after subculture on medium containing MS ＋ 2mg/L 6 -BA ＋ 0.2mg/L NAA, and each one was about three buds in average. In general, the culture medium of strong plantlets and rootage was 1/2 MS ＋ 0.2 mg/L IBA.

关 键 词：蔓生百部 组织培养 快速繁殖 

分 类 号：Q949.718.22[生物学—植物学] Q943.1