板蓝根有效部位F_(022)对脂多糖刺激小鼠组织膜结构伸展刺突蛋白mRNA表达的影响  

Effect of Component F_(022) in Radix Isatidis on the Expression Upregulation of Moesin mRNA in Mice Induced by Lipopolysaccharide

作　　者：刘云海[1] 谢委[1] 方建国[1] 李敬[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院药学部,武汉430030

出　　处：《医药导报》2006年第1期1-3,共3页Herald of Medicine

基　　金：国家自然科学基金资助项目(基金编号:39170877;39870872);卫生部科学研究基金资助项目(基金编号:98-2-110)

摘　　要：目的探讨板蓝根抗内毒素活性部位F022对脂多糖(LPS)刺激小鼠组织膜结构伸展刺突蛋白mRNA(moesin mRNA)表达的影响。方法实验前1周用卡介苗腹腔注射小鼠0.2 mL.(20 g)-1,30只小鼠平分为5组,药物实验组分别灌胃给予1.00%,0.50%和0.25%F022液0.4 mL.(20 g)-1,阴性对照组灌胃给予1.00%F022液0.4mL.(20 g)-1,LPS模型组灌胃给予同等量0.9%氯化钠溶液。1.5 h后重复给药1次。第2次给药后30 m in,药物实验组、LPS模型组小鼠尾静脉注射LPS 0.2 mL.(20 g)-1,9 h后小鼠乙醚麻醉,取肝、脾、肾组织作moesin mRNA原位杂交处理,显微镜下观察胞浆被染色情况。结果组织胞浆着色呈棕黄色者为阳性。LPS可致小鼠肝、肾、脾组织moesinmRNA表达增加,而预先给予板蓝根F022部位者moesin mRNA表达被抑制,且有剂量依赖特征。结论板蓝根F022部位对脂多糖致小鼠肝、肾、脾组织moesin mRNA表达有抑制作用。Objective To probe into the effect of the anti-endotoxic part in Radix lsatidis on the expression ot moesin mRNA in tissues of mice induced by lipopolysaccharide （ LPS ）. Methods One week before the test, an intraperitoneal injection of 0.2 mL · （20 g）^-1 bacillus calmettc-guerin（BCG） was given to each of 30 mice, which were then randomized equally into5 groups. Mice of the test groups were given 0.4 mL · （20 g）^-1 of 1.00%,0.50% and 0. 25% solution of F0z2 by gastrogavage , mice of the control group were given 0.4 mL · （20 g）^-1 of 1.00% solution of F022 by gastrogavage , and mice of the model group were given each an equal volume of 0.9% sodium chloride. The above procedures were repeated 1.5 h later, and 30 rain following the second drug administration, the test groups and the model group were given 0.2 mL · （20 g）^-1 of LPS. Mice were anesthetized 9 h later by ether with the liver, kidney and spleen tissues treated by moesin mRNA hybridization in Situ and the staining of cytoplasm observed under microscope. Results A brown yellow staining of the tissue cytoplasm was taken as positive, and the administration of LPS could increase moesin mRNA expression. With prior administration of F022 part in radix isatidis the moesin mRNA expression by LPS was inhibited with a dose dependent characteristic. Conclusion The F022 part in radix isatidis can inhibit the LPS induced moesin mRNA expression in tissues of mice liver, kidney and spleen with an antiendotoxic mechanism possibly at the molecular level.

关 键 词：板蓝根 内毒素 脂多糖 膜伸展刺突蛋白mRNA 

分 类 号：R282[医药卫生—中药学] R965[医药卫生—中医学]