功能性Cu-PVK纳米微球与核酸相互作用的共振光散射光谱及分析应用  

Resonance light scattering spectra caused by interaction of Cu-PVK nanoparticles with nucleic acids and its analytical application

作　　者：周运友[1] 佘世科[1] 刘春[1] 赵成安[1] 卢琴[1] 王伦[1] 

机构地区：[1]安徽师范大学化学与材料科学学院,芜湖241000

出　　处：《分析试验室》2005年第12期24-28,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20375001);安徽省自然科学基金(03044904)资助项目

摘　　要：在超声辐射下,H2O2氧化降解聚乙烯醇(PVA),制得与Cu2+形成配位体的纳米尺寸的β-二酮型高分子微球(Cu-PVK)。对Cu-PVK的共振散射光(RLS)性质研究发现Cu-PVK与核酸形成缔合物时将导致Cu-PVK本身RLS信号急剧增加,基于此建立一种用RLS信号测定痕量核酸的新方法。方法的抗干扰能力强,已用于合成样品的分析。A kind of functional copper-β-diketone complex nanoparticle （Cu-PVK） was prepared by an oxidation and degradation method under ultrasonic radiation. The shapes of these nanoparticles were examined by TEM to be spherical in the average diameter of 28 nm. A new method for the determination of trace nucleic acid was established based on the intensity enhancement of resonance light scattering （RLS） of Cu-PVK by the interaction of Cu-PVK with nucleic acids. This method can tolerate the interference of various substances such as various metal ions, amino acids, proteins, and surfactants and has been used to analyze the synthetic samples with satisfactory results.

关 键 词：核酸 高分子纳米粒子 聚乙烯醇 Β-二酮 共振散射光 

分 类 号：O657.31[理学—分析化学]