周期性牵张面颌致成肌细胞内Ca^(2+)浓度变化的研究  

Changes of intracellular calcium of cultured myoblast induced by cyclic stretch

作　　者：车晓霞[1] 罗颂椒[2] 李小玉[3] 

机构地区：[1]首都医科大学北京口腔医院正畸科,北京100050 [2]四川大学华西口腔医学院正畸科,成都610041 [3]四川大学华西口腔医学院细胞生物学实验室,成都610041

出　　处：《口腔医学》2005年第6期321-323,共3页Stomatology

基　　金：国家自然科学基金资助项目(30171024)

摘　　要：目的观察牵张引起的成肌细胞内Ca2+浓度变化,探求口腔正畸功能矫形治疗中肌肉改建机制。方法采用四点弯曲加力装置,使体外培养的SD大鼠面颌部成肌细胞发生牵张变形后,测定加力后不同时段及在镜下给予乙酰胆碱(Ach)后成肌细胞内Ca2+荧光强度的变化。结果牵张引起的成肌细胞内Ca2+荧光强度的升高明显高于Ach的作用,并且在经历了体外的牵张加力后,Ach刺激并未使成肌细胞内Ca2+荧光强度出现预先设想的叠加效果,在各个不同的实验组中Ca2+荧光强度反而略有下降。结论牵张引起的骨骼肌细胞内Ca2+浓度升高的机制不同于肌肉兴奋收缩耦联过程中出现的Ca2+浓度升高,Ca2+作为第二信使引发的细胞内各种结构和功能蛋白的改变可能参与了肌肉改建过程。Objective To elucidate the effects of stretch and aeetyleholine on the intraeellular calcium of myoblast and study the remodeling reaction of muscle in a variety of mechanical environments. Methods Myoblasts from maxillofaeial skeletal muscle of one week 01d male Sprague-Dawley rats were cultured and stretched eyeliely using a four-point bend device. Fluo-3 as the Ca^2＋ indicator was used to determine the intracellular calcium concentration. Acetylcholine was added to the cultured liquid under the microscope. Results The elevation of the intraeellular calcium induced by stretch was significantly higher than induced by aeetyleholine and there was no additional effect on the intraeellular ealeinm when aeetyeholine was applied to the stretched myoblast. Conclusion The results suggested calcium as a signal molecule was involved in the muscle remodeling process induced by stretch.

关 键 词：CA^2+ 大鼠 成肌细胞 周期性牵张 乙酰胆碱 

分 类 号：R445.9[医药卫生—影像医学与核医学] R329.2[医药卫生—诊断学]