盐度胁迫对许氏平鲉血液免疫酶活力的影响  

Effects of salinity on the non-specific immuno-enzymetic activity of Sebastes schlegeli

作　　者：王晓杰[1] 张秀梅[1] 李文涛[1] 

机构地区：[1]中国海洋大学教育部海水养殖重点实验室,青岛266003

出　　处：《海洋水产研究》2005年第6期17-21,共5页Marine Fisheries Research

基　　金：国家自然科学基金项目(30070593);山东省自然科学基金项目(Y2002D10)共同资助

摘　　要：研究了急性、慢性盐度胁迫对许氏平鲉体重及血液免疫相关酶活力的影响。慢性盐度胁迫实验表明,在盐度5的水体中,许氏平鲉体重增长率为负值,其余各盐度组(10、20、40)的生长指标与自然海水组(盐度33)差异不显著(P>0·05);随海水盐度的降低,许氏平鲉的溶菌酶活力逐渐上升,但当盐度降至5时,其活力与自然海水组无显著性差异;血液中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活力随海水盐度降低呈逐渐上升趋势。急性盐度胁迫实验证实,在盐度5、10的急性胁迫初期,许氏平鲉血液的各项免疫酶活力波动较大。溶菌酶活力在胁迫24h时达到峰值,之后逐渐下降;血液SOD活力在96h检测过程中呈高低起伏变化趋势;血液CAT活力在胁迫初期持续降低,12h后逐步稳定在较低水平,显著低于胁迫前血液CAT活力(P<0·05)。The acute and chronic salinity stresses on the non-specific immuno-enzymetic activity of Sebastes schlegeli were studied. In the chronic experiment, the increase rate of the fish weight was negative at salinity 5. At salinity 10, 20, 33 and 40, no significant differences in the increase rate were observed. The activity of lysozyme gradually increased as the salinity reduced from 33 to 10, but showed no significant difference between the fish at salinity 5 and 33. The activities of SOD and CAT showed a stepwise increase with the reduction in salinity. In the acute experiment, significant changes in the immunity indices were observed in the fish blood in hypoosmotic water （salinity 5 and 10）. The activity of SOD fluctuated during 96 hours and the activity of CAT continuously decreased and then became stable at a significantly low salimity level after 12 hours （P〈0.05）. At 24 h,the activity of lysozyme reached the peak,followed by a slow decrease.

关 键 词：许氏平鲉 盐度胁迫 溶菌酶 超氧化物歧化酶 过氧化氢酶 

分 类 号：S917[农业科学—水产科学]