人工选育中国对虾两个群体WSSV感染相关免疫与生化因子的变化  

Changes of immune and biochemical factors in two selected populations of prawn (Fenneropenaeus chinensis) infected by white spot syndrome virus

作　　者：刘庆慧[1] 黄倢[1] 杨冰[1] 刘莉[1] 

机构地区：[1]中国水产科学研究院黄海水产研究所,青岛266071

出　　处：《海洋水产研究》2005年第6期22-27,共6页Marine Fisheries Research

基　　金：国家自然基金项目(30271020);中国水产科学研究院项目(2003-4-1)共同资助

摘　　要：通过分析中国对虾人工选育两个群体WSSV感染相关免疫与生化因子的变化,研究选育的两个中国对虾群体对WSSV的敏感性和抵抗力。结果表明,两个群体感染WSSV后,总细胞数(THC)在24h达到最大,随后呈下降趋势;两个群体血淋巴蛋白在感染初期和中期变化不同,但在后期均呈下降趋势;相比而言2^(#)对虾群体比6^(#)对虾群体血蛋白含量和THC下降幅度稍慢;2^(#)对虾群体和6^(#)对虾群体感染24h后酸性磷酸酶(ACP)稍有下降,随后保持较高的活性,而碱性磷酸酶(AKP)的变化除2^(#)对虾群体在感染48h有显著增大外,6^(#)对虾群体变化不明显;过氧化氢酶(CAT)、活性氧(ROS)、超氧化物歧化酶(SOD)与WSSV感染有密切的联系,两个群体抗氧化酶活性随WSSV增殖的变化略有不同;细胞内酚氧化酶(proPO)原变化趋势也表现出差异,斑点杂交结果显示6^(#)对虾群体比2^(#)对虾群体阳性反应较早,揭示不同群体间对WSSV感染的敏感性存在一些差异。After infecting prawn （Fenneropenaeus chinensis） with white spot syndrome （WSSV） , we analyzed the hematological and biochemical changes in hemolymph of two selected populations of prawn and studied the sensibility and resistance of prawn to WSSV. The result showed that total haemocyte cell （THC） reached highest after 24 h infection and subsequently decreased in two populations of prawn. But there was change of hemolymph proteins in primary and middle stages of infection and decreased in the last period. Comparing two populations of shrimp, THC and hemolymph protein decreased a little more slowly in 2＇ population than those in 6＇ population. Acid phosphatase（ACP）reduced a little after 24 h infection and then kept high activity between two populations. There was insignificant change of alkalinephosphatase（AKP） except for a significant increase after 48h infection in 2-population. Correlativity was showed between WSSV infection, catalase activity, reactive oxygen and SOD activity. Anti-oxidase activity level and prophenoloxidase （proPo） activity in cell were different between two populations. Moreover, dot-hybridization result showed that positive reaction appeared earlier in 6＇ population than that in 2＇ population. There was difference in regard to infectivity and sensibility to WSSV in two populations of prawn.

关 键 词：中国对虾 选育群体 WSSV感染 免疫因子 生化因子 

分 类 号：Q7[生物学—分子生物学] R392.11[医药卫生—免疫学]