静息细胞连续两批次生物催化生产氢化可的松  

Bioconversion of compound S into hydrocortisone using a novel process of twi-run sequence bioconversion by the mycelium of Curvularia lunata AS 3.4381

作　　者：易奎星[1] 杨亚力[2] 杨顺楷[2] 肖勇[2] 王忠彦[1] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]中科院成都生物研究所,成都610041

出　　处：《生物加工过程》2005年第4期40-44,共5页Chinese Journal of Bioprocess Engineering

基　　金：云南丽江映化集团药业公司资助

摘　　要：以新月弯孢霉(Curvularia lunata)的Ⅱ级培养18 h的活菌丝作为C11β位羟基化生物催化剂,在选定的含有微粒结晶甾体底物(0.2%,质量分数)17α,21-二羟基孕甾-4-烯-3,20-二酮的磷酸缓冲液(0.05 mol/L,pH 6.0)介质体系中,在经过连续两批次约55 h的转化反应,底物转化率可维持在较高水平,产物氢化可的松净收率可达60%;从菌丝回收的底物RS可再利用。此工艺提高了生物催化剂的利用率,具有工业应用价值。Through a two-stage incubation, the mycelia of Curvularia lunata AS3.4381 grown for 18 h were harvest as a biocatalysts for C11β-hydroxylation, which were added to a phosphate buffer（0.05 moL/L, pH 6.0） system containing the microcrystal particles of 17-hydroxy-pregn-4-en-3, 20-dion-21-al-hydrate （compound S） as substrate with concentration of 0.2% （w/v） in this media. The bioconversion was conducted by a Two-Run process in a 2 L fermenter. The results showed that the conversion ratio kept on a high level in 55 h, and the net yield of hydrocortisone reached 60% （HC/RS, w/w）. In addition, the recovery of remained compound S from waste mycelia could be reused.

关 键 词：新月弯孢霉 氢化可的松 连续两批次生物转化 化合物S C11β-羟基化 

分 类 号：R977.1[医药卫生—药品]