转录因子OPBP1和OsiWRKY基因的超表达提高水稻的耐盐及抗病能力  

Overexpression of Two Different Transcription Factors, OPBP1 and OsiWRKY, Enhances Resistance Against Pathogen Attack and Salt Stress in Rice

作　　者：李南羿[1] 郭泽建[2] 

机构地区：[1]浙江大学生物技术研究所 [2]中国农业大学农学与生物技术学院,北京100094

出　　处：《中国水稻科学》2006年第1期13-18,共6页Chinese Journal of Rice Science

基　　金：国家自然科学基金资助项目(30070493);国家重点基础研究项目(G2000016203)。

摘　　要：采用基因枪法将耐盐的OPBP1和抗病相关的Osi WRKY转录因子共转化入水稻材料秀水11,经PCR及分子杂交分析确认目的基因已整合到水稻基因组中并超量表达。抗病性测定表明,与未转基因水稻相比,转基因水稻植株表现出对稻瘟病菌(Magnaporthe grisea)和白叶枯病菌(Xanthomonas oryzaepv.oryzae)的较好抗性。抗盐能力检测结果表明,在同等盐胁迫条件下转基因株系生长较快,叶绿素含量和生物学产量都显著高于未转基因对照。上述结果表明,OPBP1和Osi WRKY基因在水稻中增强表达可以提高水稻的耐盐及抗病能力。Two transcription factors, OPBP1 and OsiWRKY, involved in the regulation of signal transduction pathways under salt stress and disease resistance, respectively, were co-transformed into japonica rice Xiushui 11 by particle bombardment. PCR amplification, Southern blot and Northern blot analyses indicated that OPBP1 and OsiWRKY had been integrated into rice genome and overexpressed in rice. Compared with non-transgenic plants, transgenic lines exhibited high resistance to Magnaporthe grisea and Xanthornonas oryzae pv. oryzae, and showed improved resistance to salt stress. Under the same salt stress conditions, transgenic plants grew faster, had significant higher biomass and chlorophyll content compared to non-transgenic plants. Furthermore, it was found that higher expression level of the transgene had higher salt tolerance and disease resistance. These results suggested the overexpression of OPBP1 and OsiWRKY enhanced resistance to salt stress and pathogens in rice.

关 键 词：转基因 水稻 耐盐性 抗病性 转录因子 

分 类 号：S511.22[农业科学—作物学]