小鼠柯萨奇B_3病毒性心肌炎急性期心肌收缩力的改变及芪芩号对其影响  

The alteration of myocardial contractility in the acute phase of Coxsackievirus B_3 viral myocarditis in mice and the effect of QiqinⅠon it

作　　者：黄莹[1] 张国成[1] 杨欣伟[1] 丁翠玲[1] 胡佩珍[2] 

机构地区：[1]第四军医大学西京医院儿科,西安710032 [2]第四军医大学西京医院病理科

出　　处：《陕西医学杂志》2006年第1期8-11,41,共5页Shaanxi Medical Journal

摘　　要：观察小鼠柯萨奇B3（CVB3）病毒性心肌炎（VMC）急性期心肌收缩力的改变、采用并观察芪芩Ⅰ号（Qiqin Ⅰ）对CVB3-VMC小鼠心脏泵功能的改善。方法：采用BALB／C鼠建立CVB3病毒性心肌炎的动物模型，分为治疗组、病毒组和正常组，观察对比各组病变小鼠的一般情况、心脏收缩力指标LVP和LVdp／dtmax变化、光镜下病理积分及电镜下心肌超微结构的改变。结果：病毒组笫5、7天LVP和LVdp／dtmax两指标均明显下降，与正常组比较有极显著性差异（P〈O．01）。治疗组至笫7天两指标均开始下降，低于正常组；与病毒组相比较，其LVP在笫5、7天升高（P〈0．05），LVdp／dtmax在各时间点均有增高（P〈0．01）。光镜下治疗组的心肌病理积分在第3～7天均低于病毒组（P〈0．01）。电镜下可见病毒组小鼠心肌细胞线粒体肿胀、嵴断裂，肌丝溶解，肌浆网扩张，并在其中发现有病毒样颗粒；治疗组病变程度轻。结论：在急性期CVB3-VMC小鼠的心肌收缩力明显下降，与病毒直接损伤心肌，线粒体受损、大量心肌炎症坏死有关。芪芩Ⅰ号能明显减轻心肌的病理损害，提高CVB3-VMC小鼠的心肌收缩力。Objective：To observe the alteration of myocardial contractility in the acute phase of Coxsackievirus B3 viral myocarditis in mice, and the effect of Qiqin I for the pump function of heart on CVB3-VMC in mice. Methods： CVB3-VMC BALB/C mice were divided into treated group,virus group and control group, then the viabilities and the changes of LVP and LVdp/dtmax, pathological changes under light microscope and electron microscope and myocardial pathological scores of CVB3-VMC mice of different groups were recorded. Results..Conpared with control group both LVP and LVdp/dtmax of virus group decreased significantly at the 5th day and 7th day（P〈0. 01）,and in treat group LVP and LVdp/ dtmax decreased significantly at the 7th day. Compared with virus group, LVP and LVdp/dtmax of treated group increased significantly at different time point （P〈0. 05）. In myocardium of virus group under electron microscope, the infiltrating cells and the lysosomes in macrophagecytes and in cadiocytes were founded; mitochondria swelling, mitochondrial crista rupture, sarcoplasmic reticulum dilatation and virus-like particle in sarcoplasmic reticulum. But the changes of treated group were lighter. Conclusion： The LVP and LVdp/dtmax of CVB3-VMC BALB/C mice decreased significantly in acute phase is related with viral myocardial leision, the damage of mitochondria cadiocytes. Qiqin I not only could relieve myocardial histopathologic changes but also could improve cardiac hemodynamics in VMC mice.

关 键 词：心肌炎/病理生理学 心肌炎/病理学 心肌炎/药物疗法 柯萨奇病毒感染小鼠 

分 类 号：R259.422.1[医药卫生—中西医结合] R541.6[医药卫生—中医内科学]