豚鼠海马神经干细胞与两种不同载体材料的生物相容性  

Biocompatibility between two different biomaterials and cultured hippocampus-derived neural stem cells in vitro

作　　者：郭宝凤[1,2] 董明敏[1] 郭漳生[3] 

机构地区：[1]郑州大学第一附属医院耳鼻咽喉科 [2]武警河南总队医院耳鼻咽喉科 [3]郑州大学基础医学院解剖学教研室,河南郑州450052

出　　处：《第四军医大学学报》2006年第1期59-62,共4页Journal of the Fourth Military Medical University

基　　金：国家教育部"十五""211"工程重点学科"肿瘤与生物工程"项目[教重字(2002)第2号]

摘　　要：目的:评价豚鼠海马神经干细胞与2种不同载体材料(胶原蛋白海绵和明胶海绵)的生物相容性,探讨它们作为周围神经组织工程载体材料的可行性.方法:体外培养新生豚鼠海马神经干细胞,传至第3代,将密度为1×1010/L细胞分别与2种载体材料联合体外培养,1wk后取材,进行细胞计数,倒置相差显微镜及扫描电镜观察,并测定2种材料与细胞的吸附率.结果:神经干细胞可以在胶原蛋白海绵和明胶海绵上生长,逐渐黏附,细胞吸附率分别为37.17%和14.87%,统计学分析有显著差异;各实验组与对照组之间细胞总数统计学分析无显著差异.结论:胶原蛋白及明胶2种材料尤其是胶原蛋白,具有良好的神经干细胞相容性,可以作为周围神经组织工程的支架材料应用于临床.AIM： To study the biocompatibility between two different biomaterials （collagen protein sponge and glutin sponge ） and cultured hippocampus-derived neural stem cells in vitro and to identify whether the materials could be used as scaffold biomaterials in peripheral neural tissue engineering. METHODS： Hippocampus-derived neural stem cells of guinea pigs were cultured and respectively combined with each of the two biomaterials in vitro. The number of cells was counted and the histological changes were observed with HE staining and scanning electron microscope after 7 d and the adhesion rates were detected. RESULTS： Hippocampus-derived neural stem cells attached to both biomaterials and normally grew. The adhesion rates were 31. 17% and 14.87% respectively. The total numbers of the cells in the experimental and control groups were similar and there was no statistical difference. CONCLUSION： Both biomatcrials have a good biocompatibility with hippocampus-derived neural stem cells of guinea pigs and can be used safely as scaffold materials in peripheral neural tissue engineering.

关 键 词：神经干细胞 组织工程 生物材料 生物相容性 豚鼠 

分 类 号：R318.08[医药卫生—生物医学工程]