康莱特注射液对癌症痛大鼠痛行为和肿瘤生长的影响  

Effects of Kanglaite Injection on the Pain Behaviors and Tumor Growth in a Rat Model of Cancer-induced Bone Pain

作　　者：谭煌英[1] 高福云[2] 崔建[1] 武晓勤[1] 刘国玲[2] 李圆[1] 李佩文[1] 

机构地区：[1]中日友好医院,北京100029 [2]中日友好医院临床医学研究所中心实验室,北京100029

出　　处：《中国中医药信息杂志》2006年第1期39-42,共4页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的利用癌症痛动物模型,研究和评价康莱特注射液治疗癌症疼痛的疗效。方法根据Medhurst方法,建立大鼠乳腺癌骨痛模型。实验分康莱特组、塞来昔布组、吗啡组、生理盐水组和假手术组。从造模第8天开始给药,每日2次,连用10d。康莱特组,腹腔注射10mL/kg;塞来昔布组,30mg/kg灌胃;生理盐水组和假手术组,腹腔注射NS10mL/kg。吗啡组在造模第17天,单次腹腔注射3mg/kg。在末次给药后测量各组大鼠的痛相关行为,包括触诱发痛(大鼠足底皮肤对2gvonFrey纤毛刺激的缩足反应敏感性增强)和机械性痛觉过敏(两侧后肢负重差异)。并将大鼠后肢进行X线摄片,影像学评估肿瘤诱导的骨破坏。结果造模第17天,康莱特组、塞来昔布组、吗啡组和生理盐水组大鼠左侧足底皮肤对2gvonFrey纤毛刺激的缩足反应百分率分别为53.3%±20.7%、46.7%±20.7%、13.3%±16.3%和90%±16.7%,与生理盐水组比较,康莱特组缩足反应百分率明显下降(P<0.01);两后肢负重差异值康莱特组、塞来昔布组、吗啡组和生理盐水组大鼠分别为(48.6±28.52)g、(42.95±29.45)g、(25.81±16.99)g和(78.01±19.08)g。与生理盐水组比较,康莱特组两后肢负重差异明显变小(P<0.01)。胫骨X光照片显示,生理盐水组和吗啡组骨质破坏严重,放射学评分范围4-5;康莱特组和塞来昔布组,骨破坏程度较轻,放射学评分下降;假手术组大鼠胫骨未见放射性改变(评分均为0)。结论康莱特注射液在大鼠乳腺癌骨痛模型上有效,能减轻癌性骨痛模型大鼠痛行为,同时骨破坏程度减轻,肿瘤生长受抑。Objective To study and evaluate the effects of Kanglaite Injection （KLT） in rat model of cancer pain. Methods According to Medhurst＇ method, a rat model of cancer-induced bone pain was established by intra-tibial injection of 3 × 10^3 MRMT-1 rat mammary gland carcinomal cells in Sprague-Dawley rats. KLT group were treated with 10 mL/kg of KLT, ip, twice daily from day 8 to day 17 after injection of MRMT-1 cells. In celecoxib group, 30 mg/kg, ig, twice daily for 10 days. NS group and sham group were given with 10 mL/kg of NS, ip, twice daily for 10 days. On day 17, rats in morphine group received a single administration of 3 mg/kg morphine. Painrelated behaviors including allodynia （the hind paw withdrawal response to 2 g von Frey filament stimulation） and the difference of weight bearing between hind paws were measured in all 5 groups on day 17. In addition, the left hind limbs were removed and X-rays were obtained for assessing of tumor-induced bone destruction. Results On day 17 after injection of MRMT-1 cells or NS, the paw withdrawal response frequency to 2 g yon Frey filament stimulation on the left paw in KLT group, celecoxib group, morphine group and NS group were 53.3%±20.7%, 46.7%±20.7%, 13.3%±16.3% and 90%±16.7%, respectively. The paw withdrawal response frequency in KLT group was significantly decreased compared with that in NS group （P〈0.01）. The difference of weight bearing between hind paws in KLT group, celecoxib group, morphine group and NS group were （48.6 ± 28.52）g, （42.95 ± 29.45）g, （25.81 2 16.99）g and （78.01± 19.08）g, respectively. Weight bearing difference （g） in KLT-treated rats was significantly smaller than that in NS-treated rats （P〈0.01）. X-rays of the tibiae showed that tumor-induced bone destruction in both KLT group and celecoxib group was less severe than that in NS or morphine group. No radiologlcal change was observed in sham group. Conclusions Treatment with KLT is effective in the rat model of cancer-induced bone pain. KLT can at

关 键 词：癌症痛 动物模型 康莱特注射液 中医药疗法 

分 类 号：R273.42[医药卫生—中西医结合]