激活T细胞负性共刺激信号对大鼠同种异体肾移植存活的影响  

Influence of PDL1Ig gene transfection on the survival of renal allografts.

作　　者：冯嘉瑜[1] 张艮甫[1] 黄赤兵[1] 王平贤[1] 范明齐[1] 

机构地区：[1]第三军医大学新桥医院,重庆400037

出　　处：《四川医学》2006年第1期4-5,共2页Sichuan Medical Journal

基　　金：国家自然科学基金资助项目(30200264);国家自然科学基金重点项目(3993430-2)

摘　　要：目的观察激活负性共刺激信号程序性死亡因子及配体(PD1-PDL1)对延长移植肾存活的效能。方法以PDL1Ig基因重组腺病毒为载体,将PDL1Ig基因转入BN大鼠供肾,以Lewis大鼠为受体,行同种肾移植为转染组,并以未转染BN供肾移植给Lewis受体为对照组;观察移植肾存活时间和术后肾功能变化。结果转染组移植肾存活(36±7.3)d,较对照组移植肾存活时间(8.6±1.2)d明显延长;移植组术后血清肌酐较同期对照组明显为低。结论PDL1基因局部转染可明显延长移植肾存活时间。Objective To observe the efficacy of the hypothesis that stimulating programmed death 1 （PD-1 ）-triggered negative signals on the survival of renal allografts. Methods The kidneys of BN mrs were transfeeted with PDLI Ig gene-recombined adenovirus. The transfeeted kidneys were transplanted to Lewis rats（ transfeetion group）. BN→Lewis kidney transplantation with non-transplanted kidneys served as controls.The allograft survival time and the allograft function between the two groups were compared.Results The allograft survival time of transfection group was loger than that of controls significantly （36 ± 7.3d vs 8.6 ± 1.2d, P 〈 0.05） .The serum creatinine of transfection group at 8th postoperative days were lower than that of controls at 8th postoperative day. Conclusion Local transfection of PDLIIg gene can prolong the survival time of renal allograft significantly.

关 键 词：程序性死亡因子1 基因治疗 肾脏移植 共刺激信号 

分 类 号：R699.2[医药卫生—泌尿科学]