小麦—簇毛麦6VS/6AL易位材料幼胚一步成苗培养技术集成  

On One-step Culture Technology System Integrated for Immature Embryos of Triticum Aestivum-Haynaldia Villosa 6VS/6AL Translocation Materials

作　　者：陈升位[1] 张琼仙 毛孝强[1] 刘思严[1] 王晓丹[1] 

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]云南省监狱管理局农业科学研究所,云南昆明650600

出　　处：《云南农业大学学报》2006年第1期20-23,31,共5页Journal of Yunnan Agricultural University

基　　金：江苏省自然科学基金资助

摘　　要：基因型差异是影响小麦幼胚一步成苗培养的主要因素。比较了小麦—簇毛麦易位材料与扬麦5号和中国春的一步成苗培养的污染率、成苗率、再生植株生根培养效率等主要技术指标,建立了适用于小麦—簇毛麦易位材料幼胚的—步成苗培养的有效技术体系,为培养纯合的普通小麦—簇毛麦6VS/6AL,小片段易位体系提供了技术支持。The difference from the gene type of wheat is an importance factor effecting on the one-step culture for immature embryos of wheat. In the discourse, we compared some indicators about the one- step culture technology for immature embryos of wheat, the percentage of the amount of immature embryos polluted to total of immature embryos cultured, the percentage of amount of regenerated plantlet to total of the immature embryos cultured and the effect of culturing for the root growth of regenerated plantlet. A technological system integrated had been designed too. It is useful and valid technological system for culture for immature embryos from Triticum aestivum-Haynaldia villosa 6VS/6AL translocation materials, and can bring a help to the culture for immature embryos of wheat material with little segment of chromosome translocated from Haynaldia villosa to common wheat.

关 键 词：小麦一簇毛麦6VS/6AL易位材料 幼胚 一步成苗培养 有效技术集成 

分 类 号：S512.103.2[农业科学—作物学]