高效毛细管电泳法同时测定苦豆子总碱注射液中3种生物碱的含量  

Simultaneous Determination of Three Alkaloids in Compound Kudouzhi Injection by Capillary Electrophoresis

作　　者：罗兴平 [1] 聂凌云 [2] 翟宗德 [3] 徐培元 [4] 陈立仁 [1] 李永民 [2] 

机构地区：[1]中国科学院兰州化学物理研究所,甘肃,兰州,730000,中科院研究生院,北京,100037,甘肃省药品检验所,甘肃,兰州,730000 [2]中国人民解放军总后勤部卫生部药品仪器检验所,北京,100071 [3]中国科学院兰州化学物理研究所,甘肃,兰州,730000,中科院研究生院,北京,100037 [4]甘肃省药品检验所,甘肃,兰州,730000

出　　处：《解放军药学学报》2005年第6期458-460,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的应用高效毛细管电泳法测定苦豆子总碱注射液中苦参碱、氧化苦参碱、槐定碱的含量。方法运行缓冲液为100mmol·L-1硼砂,pH=9.2;运行电压8kV(恒压分析);柱温25℃;检测波长215nm;盐酸麻黄碱为内标。结果槐定碱、苦参碱、氧化苦参碱的线性范围分别为52.4～524、48.8～488、20.4～204μg·ml-1,平均回收率分别为98.22%、97.73%、98.82%,RSD分别为1.38%、2.15%、1.99%(n=6)。结论该方法简便、准确、重现性好,可作为苦豆子总碱注射液中生物碱的质量控制方法。Aim To establish an HPCE method for the simultaneous detennination of sophoridine, matrine and oxymatrine in compound kudouzhi injection. Method The separation was achieved with 100mmol·L^-1 sodium borate （pH = 9. 2） as buffer solution at a constant voltage of 8kV and temperature at 25℃. The detective wavelength was 215 nm. Ephedrine was used as the internal standard. Results The linear detennination ranger was 52.4 - 524μg·ml^-1 for sophordine, 48.8 - 488μg·ml^-1 for matrine and 20.4 - 204μg·ml^-1 for oxymatrine. The recoveries and the RSD were 98. 22% ,1.38% for sophoridine, 97.73%, 2.15% for matrine and 98.82%, 1.99% for oxymatrine. Conclusion The method is simple and accurate and can he used for quality control of compound kudouzhi injection.

关 键 词：高效毛细管电泳 苦豆子总碱注射液 苦参碱 氧化苦参碱 槐定碱 

分 类 号：R917[医药卫生—药物分析学]