龙眼体细胞胚胎发生过程的基因差异表达  

Diferential Gene Expression in the Process of Somatic Embryogenesis in Longan(Dimocarpus longan Lour.)

作　　者：王凤华[1] 赖钟雄 郑金贵 吕柳新[3] 

机构地区：[1]福建农林大学亚热带果树研究所,河南科技大学林学院洛阳471003 [2]福建农林大学作物科学学院 [3]福建农林大学园艺学院,福州350002

出　　处：《农业生物技术学报》2005年第6期703-708,共6页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(No.30471204);福建省自然科学基金(No.B0010014和C0410012);霍英东教育基金会高校青年教师基金(No.71026);教育部高等学校首批骨干教师项目资助

摘　　要：采用mRNA差别显示技术对龙眼(DimocarpuslonganLour.)体细胞胚胎发生过程中的胚性愈伤组织、球形胚、鱼雷胚、子叶胚、成熟胚等阶段进行分析。改进并优化了mRNA差别显示体系,获得若干差异片段,Northernblot和反Northernblot验证获得阳性片段3条:longan1、longan2和longan4。其中longan1在愈伤组织和球形胚阶段特异表达;longan2在5个阶段均表达,可能是组成型表达基因;longan4在子叶胚阶段特异表达。登录GenBank比较发现longan1与甜椒(AY129560)和番茄(AF413573)的apx基因具有较高的同源性,分别为97%和92%。。The mRNA differential display method was applied to analyse differential gene expression in the process of somatic embryogenesis in longan （Dimocarpus longan Lour.）. mRNA differential display system was improved and optimized and several differential expression cDNAs were obtained using this method at the stages of the callus, globular embryoid, torpedo-shaped embryoid, cotyledonary embryoid and matured cotyledonary embryoid. Among them the longanl was proved to be expressed at the callus stage and globular stage; the longan2 expressed at all the five stages, which was maybe a house-keeping gene; and the longan4 expressed at the cotyledonary embryoid stage. Blast results in GenBank showed that longanl was highly homologous（92% and 97%, respectively） to apx gene of tomato （AF413573） and sweet pepper （AY 129560）.

关 键 词：龙眼 体细胞胚胎发生 MRNA差别显示 基因表达 

分 类 号：S188[农业科学—农业基础科学] S512.1